The application of diagnosis and treatment of Angiopoietin and Ephrin from retinal ischemia in the angiogenesis

血管生成通过视网膜血管的循环障碍导致视网膜缺血。许多细胞因子，如血管内皮细胞生长因子，似乎与血管生成有关。为诱导视网膜缺血继发红斑和新生血管形成，用激光光凝结扎3只日本猴6只眼的视网膜主干静脉。光凝后1周、2周、4周分别摘除2只红斑眼和新生血管眼。采用免疫组织化学方法，对虹膜、小梁和视网膜新生血管石蜡切片进行免疫组织化学染色。治疗后1周、2周、4周小梁网和Schlemms管均呈阳性染色，抗体定位采用氨乙基卡巴唑显色法。治疗后2周和4周的切片中，ewitin-B2呈阳性表达，而在1周的切片中表达不明确。在所有切片中，ePhrin-B4和Angiopoietin-2的表达均不显著。治疗后1、2、4周所有视网膜新生血管均呈VEGF阳性表达。其中1例治疗后仅2周切片中EPhin-B2、B4、Angiopoietin-2呈阳性表达，另1例未见EPhin-B4、Angiopoietin-2阳性表达。在一周和四周的时间里，这两个部分的情况都不确定。在实验性红斑病和新生血管形成中，血管内皮生长因子的表达紧随其后的是EPhin-B2的表达。这些数据提示，动脉分化过程中EPhin-B2的表达可能在新生血管的生长过程中起作用。

Angiogenesis produces retinal ischemia by circulatory disturbance of retinal vessel. Many cytokines such as vascular endothelial grouth factor (VGEF) seem to be related to the angiogenesis. In order to induce rubeosis and neovascularization secondary to retinal ischemia, major retinal veins were occluded with laser photocoagulation in 6 eyes of 3 Japanese monkeys. Two eyes with rubeosis and neovascularization were enucleated one week, two weeks and four weeks respectively after photocoagulation. Immunohistochemical analysis was performed on fixed paraffin sections of the iris, trabeculum and retinal neovascularization using polyclonal antibody against Ephrin-B2, Eph-B4, Angiopoietin-2 and VEGF. Antibody localization was examined using anavidin-biotin reaction linked to aminoethylcarbabazole color reagent the trabecular meshwork and the Schlemms canal including 1, 2 and 4 weeks after treatment, showed positive staining for VEGF. Ephrin-B2 was positively stained in the section of 2 and 4 weeks after treatment but it was uncertain in the sections of one week. Ephrin- B4 and Angiopoietin-2 were not significant in any sections. All retinal neovascularization 1, 2 and 4 weeks after treatment, showed positive staining for VEGF. One case, Ephrin-B2, B4 and Angiopoietin-2 were positively stained in the section for only 2 weeks after treatment, but other case was uncertain for Ephrin- B4 and Angiopoietin-2. It was uncertain in the sections for one and four weeks. Expression of VEGF was followed by that of Ephrin-B2 in experimentally induced rubeosis and neovasucularization. The data suggests the expression of Ephrin-B2, an arterial differentiation, might play a role in grouth of newly formed vessels.