Role of earlyocular chemokines and chemokine receptors after corneal allograft

早期眼趋化因子和趋化因子受体在同种异体角膜移植后的作用

• 批准号: 12671720
• 负责人: YAMAGAMI Satoru
• 金额: $ 2.18万
• 依托单位: Jichi Medical
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671720/
• 关键词: corneal transplantation; chemokine; cervical lymph nodes; chemokine Receptor; allograft rejection; 頸部リンパ節; ランゲルハンス細胞; CCR5; 角膜; ノックアウトマウス

We investigated chemokines and chemokine receptors in corneal Langerhans cell migration and allograft rejection. We showed the over expression of RANTES, MIP-1α, MIP-1β, MCP-1 and their receptors such as CCR1, CCR2 and CCR5 mRNAs in the postoperative early as well as effectors phases of corneal transplants. Moreover, CCR1 play a critical role in generating allograft rejection by performing knockout mouse study. In Langerhans cell migration of corneal epithelium after cauterization ; CCR5 functionally affects Langerhans cell migration in cornea. These results suggest that chemokine and their receptors are closely associated with the cell recruitment in allo-recognition and allo-sensitization after corneal transplants. In addition, we studied critical role of draining cervical lymph nodes in corneal allograft rejection. The study to reveal correlation between chemokine and cervical lymph nodes/corneas is on going in our laboratory.

我们研究了趋化因子和趋化因子受体在角膜郎格汉斯细胞迁移和同种异体移植排斥反应中的作用。结果显示，RANTES、MIP-1α、MIP-1β、MCP-1及其受体CCR1mRNAs、CCR2mRNAs和CCR5mRNAs在角膜移植术后早期和效应期均有高表达。此外，通过对小鼠进行基因敲除研究，CCR1在同种异体移植排斥反应的发生中起着关键作用。烫伤后朗格汉斯细胞迁移；CCR5在功能上影响朗格汉斯细胞在角膜中的迁移。这些结果提示趋化因子及其受体与角膜移植后同种异体识别和同种异体致敏的细胞募集密切相关。此外，我们还研究了引流颈淋巴结在角膜移植排斥反应中的关键作用。我们实验室正在进行一项揭示趋化因子与颈部淋巴结/角膜的相关性的研究。

项目成果

Yamagami S: "The critical role of draining lymph nodes in corneal alloimmunization and graft rejection"Invest Ophthalmol Vis Sci. 42. 1293-1298 (2001)

Yamagami S：“引流淋巴结在角膜同种免疫和移植物排斥中的关键作用”Invest Ophasemol Vis Sci。

Yamagami S, et al: "The critical role of lymph nodes in corneal allo-immunigation and graft rejection"Invest ophthalmol Vis Sci. 42. 1293-1296 (2001)

Yamagami S 等人：“淋巴结在角膜同种异体免疫和移植物排斥中的关键作用”Invest ophamol Vis Sci。

Yamagami S: "The critical role of lymph nodes in corneal allo-immunization and graft rejection"Invest Ophthalmol Vis Sci. 42. 1293-1298 (2001)

Yamagami S：“淋巴结在角膜同种异体免疫和移植物排斥中的关键作用”Invest Ophasemol Vis Sci。

Yamagami S, et al: "Draining lymph nodes play an essential role in allo immunity generated in response to high-risk corneal transplantation"Cornea. (in press).

Yamagami S 等人：“引流淋巴结在高风险角膜移植产生的同种异体免疫中发挥着重要作用”。

Qian Y: "Topical soluble tumor necrosis factor alpha receptor type I suppresses ocular chemokine gene expression and rejection of allogeneic corneal transplants"Arch Ophthalmol. 118. 1666-1671 (2001)

钱Y：“局部可溶性肿瘤坏死因子α受体I型抑制眼部趋化因子基因表达和同种异体角膜移植的排斥”Arch Ophamol。