Experimental study on anti-oncogene and oncogene in eye disorders

抗癌基因和癌基因在眼部疾病中的实验研究

• 批准号: 12671718
• 负责人: OHNISHI Yoshitaka
• 金额: $ 2.18万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671718/
• 关键词: ocular tumor; anti-oncogene; oncogene; activating protein 1; XPC knockout mouse; transforming growth factor; がん制御遺伝子; 癌抑制遺伝子

The purpose of this study is to clarify the role of anti-oncogene and oncogene in eye disorders.Retinoblastoma gene was examined in the patient, and p53 gene was studied on the patients with pterygium, squamous cell carcinoma, basal cell carcinoma and sebaceous carcinoma of the eyelid. The p53 gene was associated with carcinomas, but not with pterygium.The spacial and temporal expression patterns of proteins and mRNAs of the components of transcription factor activator protein 1 (AP -1) to examine the activation pattern of lens epithelial cells during lens wound healing were investigated. This study showed that mRNA and protein of AP-1 components were transiently expressed in the epithelium of rat crystalline lens in the very early phase after capsular injury, although iris constitutively expressed c-fos and c-fun.To determine whether endogenous TGF β affects lens epithelial cells during repair after an anterior capsule injury in mice, translocation of Smad proteins, which carry the TG … More F β signal from cell surface receptors to promotes in nuclei, was studied. Posttraumatic murine lens epithelial cells were regulated by endogenous TGF β as indicated by translocation of Smad into nuclei as early as 8 hours after anterior capsule injury. Injection of antibodies neutralizing TGF β 2, but not antibodies specific for TGF β 1 orTGFβ, inhibited Smad4 nuclear translocation, further indicating that the translocation of Smad had been induced by endogenous TGF β 2. Another experiment showed that human lens epithelial cells were regulated by endogenous TGF β during postoperative healing. Nuclear localization of Smads3/4 was demonstrated in postoperative lens, while these Smads were not detected in the nuclei of lens epithelial cells of uninjured lenses or in the cells of anterior capsule freshly obtained during ocular surgery.TGF β soluble type II receptor by adenovivus - mediated gene transfer effectively inhibited TGF β signaling and experimental retinal gliosis both in vitro and in vivo.Animal model of eyelid tumor was established using 9, 10 - dimethyl -1, 2- benzanthracene. Less

为探讨抑癌基因和癌基因在眼部疾病中的作用，对1例视网膜母细胞瘤患者进行了抑癌基因检测，并对翼状胬肉、眼睑鳞状细胞癌、基底细胞癌和皮脂腺癌患者进行了p53基因检测。为探讨透镜损伤愈合过程中透镜上皮细胞的激活模式，本研究通过检测转录因子激活蛋白1（AP-1）各组分的蛋白质和mRNA的时空表达模式，探讨了AP-1在晶状体损伤愈合过程中的作用。本研究表明，大鼠晶状体囊膜损伤后早期，透镜上皮细胞中存在AP-1的mRNA和蛋白的瞬时表达，而虹膜组织中表达c-fos和c-fun。 ...更多信息 研究了F β从细胞表面受体到核内启动子的信号传导。外伤后小鼠透镜上皮细胞受到内源性TGF β的调节，如早在前囊损伤后8小时Smad易位到细胞核中所示。注射中和TGF β 2的抗体，而不是TGF β 1或TGF β特异性抗体，可抑制Smad 4核转位，进一步表明内源性TGF β 2诱导了Smad 4核转位。另一项实验表明，人透镜上皮细胞在术后愈合过程中受到内源性TGF β的调节。术后透镜中Smads 3/4核定位，而在未损伤晶状体的透镜上皮细胞的细胞核或在眼手术中新鲜获得的前囊细胞中未检测到这些Smads。介导的基因转移在体内外均能有效抑制TGF β信号转导和实验性视网膜胶质增生。使用9，10 -二甲基-1，2-苯并蒽建立。少

项目成果

Okada Y, Ohnishi Y, et al.: "Disappearance of desmosomal components in rat corneal epithelium during wound healing"Opthalmologica. 215. 61-65 (2001)

Okada Y、Ohnishi Y 等人：“伤口愈合过程中大鼠角膜上皮中桥粒成分的消失”Opthalmologica。

Yoshitomi T, Ohnishi Y, et al.: "Effect of latanoprost, prostaglandin F2 α and nipradilol on isolated bovine ciliary muscle"Jpn J Ophthalmol. 46. 401-405 (2002)

Yoshitomi T、Ohnishi Y等人：“拉坦前列素、前列腺素F2α和尼普地洛对离体牛睫状肌的影响”Jpn J Ophamol. 46. 401-405 (2002)

Ohnishi Y, Yoshitomi T, et al: "Electron microscopic study of monkey retina after photodynamic treatment"Med Electron Microsc. 35-1. 46-52 (2002)

Ohnishi Y、Yoshitomi T 等人：“光动力治疗后猴子视网膜的电子显微镜研究”Med Electron Microsc。

Saika S, Ohnishi Y, et al: "Epithelial repair roles of extracellular matrix"Cornea. 21-1. S23-S29 (2002)

Saika S、Ohnishi Y 等人：“细胞外基质的上皮修复作用”角膜。

Ohnishi Y, et al.: "Electron microscopic study of monkey retina after photodynamic treatment"Med Electron Microsc. 35・1. 46-52 (2002)

Ohnishi Y等人：“光动力治疗后的猴子视网膜的电子显微镜研究”Med Electron Microsc 35・1（2002）。