Experimental study on multidisciplinary treatment for malignant tumors of the eye.

本研究的目的是开发眼部肿瘤的多学科治疗方法，包括电疗、光动力治疗和热疗。培养恶性黑色素瘤细胞。其中一些被单独暴露在不同强度的电脉冲下（500-2500 V/cm）。然后将其他细胞暴露于抗增殖剂：博来霉素，丝裂霉素C或5-氟尿嘧啶中30分钟，有或没有电脉冲。通过第3天的细胞计数和H^3胸腺嘧啶摄取试验评估细胞增殖。只有在2500V/cm时，细胞数量才有显著减少。博莱霉素、丝裂霉素C和5-氟尿嘧啶治疗在有或没有电脉冲的情况下均以剂量依赖的方式抑制细胞增殖。电脉冲处理对博来霉素细胞增殖的抑制作用是博来霉素单独作用的100倍。将培养的恶性黑色素瘤细胞移植到小鼠眼睑。待移植肿瘤细胞生长后，采用电穿孔法进行化疗。使用的药物为博来霉素、丝裂霉素C和5-氟尿嘧啶。最有效的药物是博来霉素。因此，电化学疗法可以减少治疗恶性肿瘤所需的抗增殖剂剂量。我们为青光眼手术开发了这种电疗。

The purpose of this study is to develop multidisciplinary treatments in ocular tumors, using electrochemotherapy, photodynamic therapy and hyperthermic treatment.Malignant melanoma cells were cultured. Some of these sells were exposed to various intensities of electric pulse alone (500-2500)V/cm). Other cells were then exposed for 30 min to an antiproliferative agent : bleomycin, mitomycin C or 5-fluorouracil with or without electric pulse. Cell proliferation was assessed by cell counting on day 3 and by a H^3-thymidine uptake assay. A significant reduction in the cell number was observed only at 2500V/cm. Bleomycin, mitomycin C and 5-fluorouracil treatment inhibited cell proliferation in a dose-dependent manner either with or without electric pulse. Electric pulse treatment induced an inhibitory effect of bleomycin cell proliferation which was 100 times more prominent than bleomycin alone..Malignant melanoma cells cultured were transplanted into the eyelid of the mouse. After the growth of the transplanted tumor cells, electrochemotherapy using electroporation method was performed. The drugs used were bleomycin, mitomycin C and 5-fluorauracil. The most effective drug is bleomycin for this electrochemotherapy.The electrochemotherapy may therefore reduce the necessary dose of antiproliferative agents for the treatment against malignant tumors. We developed this electrochemotherapy for glaucoma surgery.