Basic research on angiogenesis in neuroblastomas

神经母细胞瘤血管生成的基础研究

• 批准号: 12671742
• 负责人: KOMURO Hiroaki
• 金额: $ 1.28万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671742/
• 关键词: Neuroblastoma; Angiogenesis; VEGF; bFGF; PD-ECGF; 神経芽腫

The growth and metastasis of malignant tumors is largely dependent on angiogenesis. Angiogenic factors produced by tumor cells are known to promote tumor angiogenesis. The aim of this study is to investigate which angiogenic factor is the most important in the progression of neuroblastoma (NB). Procedure : The relative expression levels of vascular endothelial growth factor -A (VEGF-A), VEGF-C, basic fibroblast growth factor (bFGF), and platelet-derived endothelial growth factor (PD-ECGF/TP) were studied in 28 NB tumor specimens by real-time quantitative reverse transcriptase/polymerase chain reaction (RT-PCR). The relationships between the expression of these four angiogenic factors and stage, patient age, primary site, MYCN copy number, and lymph node metastasis were analyzed. Results: High VEGF-A expression was correlated with stage 4 disease (blood-borne metastasis). No relationship between VEGF-A expression and age, primary site, MYCN copy number, or lymph node metastasis was found. VEGF-C, bFGF, or PD-ECGF/TP expressions showed no correlation with stage, age, primary site, MYCN copy number, or lymph node metastasis. Conclusions : Our findings suggest that VEGF-A may be associated with progression of NB. VEGF-A could be a target for antiangiogenic therapy for disseminated NB.

恶性肿瘤的生长和转移在很大程度上依赖于血管生成。已知由肿瘤细胞产生的血管生成因子促进肿瘤血管生成。本研究的目的是探讨哪种血管生成因子在神经母细胞瘤（NB）的进展中是最重要的。程序：应用实时荧光定量逆转录聚合酶链反应（RT-PCR）检测了28例NB肿瘤组织中血管内皮生长因子-A（VEGF-A）、VEGF-C、碱性成纤维细胞生长因子（bFGF）和血小板源性内皮生长因子（PD-ECGF/TP）的相对表达水平。分析这4种血管生成因子的表达与肿瘤分期、患者年龄、原发部位、MYCN拷贝数和淋巴结转移的关系。结果：VEGF-A高表达与4期（血源性转移）疾病相关。VEGF-A表达与年龄、原发部位、MYCN拷贝数、淋巴结转移无关，VEGF-C、bFGF、PD-ECGF/TP表达与分期、年龄、原发部位、MYCN拷贝数、淋巴结转移无关。结论：我们的研究结果表明，VEGF-A可能与NB的进展。VEGF-A可能是抗血管生成治疗播散性NB的靶点。

项目成果

Hiroaki Komuro: "Laparoscopic resection of an adrenal neuroblastoma detected by mass-screening which grew in size during observation"Surgical Endoscopy. 14. 297 (2000)

小室弘明：“腹腔镜切除通过大规模筛查发现的肾上腺神经母细胞瘤，该瘤在观察过程中体积不断增大”外科内窥镜检查。

Hiroaki Komuro: "Expression of angiogenic faceors and tumor progression in human neuroblastoma"Journal of Cancer Research and Clinical Oncology. 127. 739-743 (2001)

Hiroaki Komuro：“人类神经母细胞瘤中血管生成面孔和肿瘤进展的表达”癌症研究和临床肿瘤学杂志。

Hiroaki Komuro: "Expression of angiogenic factors and tumor progresion in human neuroblastoma"Journal of Cancer Research and Clinical Oncology. 127. 739-743 (2001)

Hiroaki Komuro：“人神经母细胞瘤中血管生成因子的表达和肿瘤进展”癌症研究和临床肿瘤学杂志。

Komuro H et al.: "Expression of angiogenic factors and tumor progression in human neuroblastoma"Journal of Cancer Research and Clinical Oncology. 127. 739-743 (2001)

Komuro H等人：“人神经母细胞瘤中血管生成因子的表达和肿瘤进展”癌症研究和临床肿瘤学杂志。

Komuro H et al.: "Laparoscopic resection of an adrenal neuroblastoma detected by mass-screening which grew in size during observation"Surgical Endoscopy. 14. 297 (2000)

Komuro H 等人：“腹腔镜切除通过大规模筛查检测到的肾上腺神经母细胞瘤，该瘤在观察过程中体积不断增大”外科内窥镜检查。