Stem cell research in ordermade regenerative medicine for pediatric patients and application of amniotn

(1)使用胃脱细胞基质再生食管：自体食管的组织工程被认为是食管替代物的一个有前途的策略。在这项研究中，胃脱细胞基质（GAM）作为支架再生的食管在大鼠模型。GAM补片移植物提供了令人满意的食管粘膜再生，没有狭窄或扩张，尽管肌肉再生仍然是未来的挑战。（2）PLAG补片-胶原海绵混合支架在疝修补中的应用：对于先天性疝的大缺损，有时需要补片闭合。但使用假体材料可能导致肉芽形成、过敏反应或感染、疝复发和胸廓畸形。组织工程可能成为一种替代的治疗策略，修复肿瘤，因为再生的自体组织有望与受影响的婴儿一起生长，而不会产生任何排斥反应。 ...更多信息 感染或感染。作者在大鼠模型中评价了使用聚（DL-乳酸-羟基乙酸共聚物）（PLGA）补片-胶原海绵混合支架（设计用于原位组织工程）进行疝修补术的有效性。PLGA网片-胶原海绵复合支架能更好地促进自体原位组织再生。这可能适用于替代其他假体补片的血管修复。(3)大鼠羊膜上皮细胞的神经分化潜力：来自胚胎的羊膜上皮细胞（AEC）被认为是干细胞样细胞群，是再生医学的一个有吸引力的细胞来源，因为在分娩时可以非侵入性地获得大量细胞。本实验研究了大鼠AEC的神经分化潜能。神经诱导后，大鼠AEC的形状发生了显着变化，突出的树突状结构。免疫细胞化学显示，约20%的诱导细胞显示未成熟神经元标记物β III-微管蛋白的表达。大鼠AEC可能已经定向分化为各种神经谱系，并且它们可以在体外分化为未成熟的神经元。少

(1) Regeneration of the esophagus using gastric acellular matrix: Tissue engineering of the autologous esophagus has been thought to provide a promising strategy for esophageal substitution. In this study, gastric acellular matrix (GAM) was used as a scaffold for regeneration of the esophagus in a rat model. GAM patch graft provide satisfactory mucosal regeneration of the esophagus without stenosis or dilatation, although muscle regeneration was still a future challenge(2) Application of PLAG mesh-collagen sponge hybrid scaffold for diaphragmatic repair: Patch closure is sometimes required for the large defect of congenital diaphragmatic hernia. But the use of prosthetic materials may lead to induction of granulation, allergic reaction, or infection, recurrence of hernia, and thoracic deformity. Tissue engineering may become an alternative treatment strategy for diaphragmatic repair, because the regenerated autologous tissue is expected to grow with the affected infants without any rej … More ection or infection. The authors evaluated the efficacy of diaphragmatic hernia repair in a rat model using Poly (DL-lactic-co-glycolic acid) (PLGA) mesh-collagen sponge hybrid scaffold which was designed for in situ tissue engineering. The PLGA mesh-collagen sponge hybrid scaffold provided better promotion of autologous in situ tissue regeneration. This could be possibly applied to diaphragmatic repair in substitution for other prosthetic patches.(3) Neural differentiation potential of rat amniotic epithelial cells: Amniotic epithelial cells (AEC) derived from embryos are thought to represent a stem-like cell population and to be an attractive cell source for regenerative medicine, because abundant cells can be obtained non-invasively at delivery. In this study, the neural differentiation potential of rat AEC was investigated. After neural induction, rat AEC dramatically changed their shapes, projecting dendrite-like structures. Immunocytochemically, 〜20% of the induced cells showed expression of an immature neuronal marker, βIII-tubulin. Rat AEC might be already committed to differentiate to various neural lineages and that they could differentiate to immature neurons in vitro. Less