Regenerative medicine for pediatric surgical diseases using stem cells with their improved microenvironments.

高位肛门直肠畸形患儿手术后常出现大便失禁，主要原因是排便肌发育不全。我们研究了通过成肌细胞移植再生排便肌以改善排便控制的可能性。研究了成肌细胞移植联合碱性成纤维细胞生长因子(BFGF)的控制和缓释的效果。从明胶水凝胶微球中控制和持续释放碱性成纤维细胞生长因子可提高移植成肌细胞的存活率，并通过促进肌肉生成而不是血管生成来促进肌肉再生。成肌细胞移植联合生长因子可能为改善婴幼儿肛门直肠畸形术后排便控制提供一种新的治疗策略。

Infants with higher anorectal anomalies often develop fecal incontinence after surgical reconstruction mainly due to the incomplete development of defecation muscles. We investigated the possibility of defecation muscle regeneration by myoblast transplantation to improve fecal continence. The efficacy of myoblast transplantation in combination with the controlled and sustained delivery of basic fibroblast growth factor (bFGF) is investigated. The controlled and sustained release of bFGF from gelatin hydrogel microspheres improves the survival rate of transplanted myoblasts and promotes muscle regeneration by facilitating myogenesis rather than angiogenesis. Myoblast transplantation coupled with growth factors may provide a novel treatment strategy for improving fecal continence after repair of anorectal anomalies in infants.