Analysis of the candidate tumor suppressor ING1 gene in neuroblastoma.

根据ING1基因下调对软琼脂生长、体外病灶形成和体内肿瘤形成的促进作用，分离到候选抑癌基因ING1。ING1编码一系列选择性剪接的、核的、细胞周期调节的蛋白，其中一些通过转录激活p21WAF1/Cip1与P53协同负调控细胞生长。为探讨ING1是否参与神经母细胞瘤的发生发展，我们对80例原代神经母细胞瘤组织和6个细胞系进行了突变筛查，并对20例神经母细胞瘤组织和6个细胞系的ING1基因表达水平进行了研究。未检测到突变，但在所有9个原发神经母细胞瘤和6个神经母细胞瘤细胞系中ING1mRNA表达均显著降低。这些观察结果表明，ING1基因的突变在神经母细胞瘤中很少见，但提示ING1基因的下调可能参与了神经母细胞瘤的发生和发展。

The candidate tumor suppressor gene ING1 was isolated, based on the observation that down regulation og the gene promotes growth in soft agar and focus formation in vitro and tumor formation in vivo. ING1 encodes a family of alternative spliced, nuclear, cell-cycle-regulated proteins, some of which cooperate with p53 in the negative regulation of cell growth by transcriptionally activating p21WAF1/Cip1. To examine whether ING1 was involved in the development of neuroblastoma, we screened for mutation in 80 primary neuroblastoma tissues and 6 cell lines, and stdied the expression level of ING1 mRNA in 20 neuroblastoma tissues and 6 cell lines. No mutations were detected, however, marked decreases in ING1 mRNA expression were seen in all 9 primary neuroblastomas and 6 neuroblastoma cell lines examined. These observations show that mutation of the ING1 gene is rare in neuroblastoma, however, suggest that down regulation of ING1 might contribute to the genesis and development of neroblastoma.