Analysis of the candidate tumor suppressor ING1 gene in neuroblastoma.

神经母细胞瘤中候选抑癌基因 ING1 的分析。

• 批准号: 12671740
• 负责人: KONDO Satoshi
• 金额: $ 1.79万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671740/
• 关键词: ING1; tumor suppressor gene; neuroblastoma; 分子生物学; ING1遺伝子

The candidate tumor suppressor gene ING1 was isolated, based on the observation that down regulation og the gene promotes growth in soft agar and focus formation in vitro and tumor formation in vivo. ING1 encodes a family of alternative spliced, nuclear, cell-cycle-regulated proteins, some of which cooperate with p53 in the negative regulation of cell growth by transcriptionally activating p21WAF1/Cip1. To examine whether ING1 was involved in the development of neuroblastoma, we screened for mutation in 80 primary neuroblastoma tissues and 6 cell lines, and stdied the expression level of ING1 mRNA in 20 neuroblastoma tissues and 6 cell lines. No mutations were detected, however, marked decreases in ING1 mRNA expression were seen in all 9 primary neuroblastomas and 6 neuroblastoma cell lines examined. These observations show that mutation of the ING1 gene is rare in neuroblastoma, however, suggest that down regulation of ING1 might contribute to the genesis and development of neroblastoma.

基于观察到该基因的下调促进软琼脂中的生长以及体外病灶形成和体内肿瘤形成，分离了候选抑癌基因ING1。 ING1 编码一系列选择性剪接的核细胞周期调节蛋白，其中一些蛋白通过转录激活 p21WAF1/Cip1 与 p53 合作，对细胞生长进行负调节。为了检验ING1是否参与神经母细胞瘤的发生发展，我们对80个原发性神经母细胞瘤组织和6个细胞系进行了突变筛查，并检测了20个神经母细胞瘤组织和6个细胞系中ING1 mRNA的表达水平。未检测到突变，但在所有 9 个原发性神经母细胞瘤和 6 个检查的神经母细胞瘤细胞系中均发现 ING1 mRNA 表达显着降低。这些观察结果表明，ING1基因的突变在神经母细胞瘤中很少见，然而，ING1的下调可能有助于神经母细胞瘤的发生和发展。