Analysis of the candidate tumor suppressor ING1 gene in neuroblastoma.

候选抑癌基因ING1的分离是基于观察到该基因的下调在体外促进软琼脂生长和病灶形成，在体内促进肿瘤形成。ING1编码一个可选择剪接的细胞核细胞周期调节蛋白家族，其中一些通过转录激活p21WAF1/Cip1与p53合作，负向调控细胞生长。为了研究ING1是否参与神经母细胞瘤的发生发展，我们在80个原发神经母细胞瘤组织和6个细胞系中进行了基因突变筛选，研究了ING1 mRNA在20个神经母细胞瘤组织和6个细胞系中的表达水平。未检测到突变，但在所有9个原发性神经母细胞瘤和6个神经母细胞瘤细胞系中均观察到ING1 mRNA表达明显降低。这些观察结果表明，ING1基因的突变在神经母细胞瘤中是罕见的，然而，ING1基因的下调可能与神经母细胞瘤的发生和发展有关。

The candidate tumor suppressor gene ING1 was isolated, based on the observation that down regulation og the gene promotes growth in soft agar and focus formation in vitro and tumor formation in vivo. ING1 encodes a family of alternative spliced, nuclear, cell-cycle-regulated proteins, some of which cooperate with p53 in the negative regulation of cell growth by transcriptionally activating p21WAF1/Cip1. To examine whether ING1 was involved in the development of neuroblastoma, we screened for mutation in 80 primary neuroblastoma tissues and 6 cell lines, and stdied the expression level of ING1 mRNA in 20 neuroblastoma tissues and 6 cell lines. No mutations were detected, however, marked decreases in ING1 mRNA expression were seen in all 9 primary neuroblastomas and 6 neuroblastoma cell lines examined. These observations show that mutation of the ING1 gene is rare in neuroblastoma, however, suggest that down regulation of ING1 might contribute to the genesis and development of neroblastoma.