The research about overexpression gene identification with neuroblastoma on Biochip

神经母细胞瘤过表达基因生物芯片鉴定研究

• 批准号: 14571705
• 负责人: KONDO Satoshi
• 金额: $ 1.86万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571705/
• 关键词: neuroblastoma; cDNA microarray; spontaneous differentiation and regression; BioChip(microarray); 病理組織; 正常交感神経節

This research planned to fine overexpression gene of neuroblastoma by using Biochip at first. However, technically, because it was impossible, it decided to identify the gene which participates in the mechanism of spontaneous differentiation and regression with cDNA microarray.Neuroblastoma (NB), one of the most common solid tumors among children, is known found under the age of one year often show spontaneous differentiation and regression. But some shift to unfavorable type and progress to an advance stage with metastasis. Therefore, it is very important to know which tumor will regress or progress. Using histological classified by the degree of maturation, we analyzed gene-expression profiles of 14 NB tumors on coda microarray to elucidate the mechanisms of spontaneous differentiation and regression. Computational analysis identified the gene which has a difference between differentiating NB tumors and poorly differentiated NB tumors. Differentiating NB group included genes associated with cell maturation and apoptosis. Poorly differentiated NB group included in relation to tumorigenesis. The future, examination will be added ; it thinks that we want to be able to lead to development of new therapeutic strategies.

这项研究计划通过首先使用生物芯片来细胞神经母细胞瘤的过表达基因。然而，从技术上讲，由于不可能，它决定鉴定参与自发分化和与cDNA微阵列回归机制的基因。在一个年龄下，儿童中最常见的实体瘤之一的神经细胞瘤（NB）经常在一个年龄下显示出自发的差异和回归。但是，有些人转向不利的类型，并以转移的形式转向前进阶段。因此，知道哪些肿瘤会消退或进展非常重要。使用通过成熟程度进行的组织学分类，我们分析了尾声微阵列上14个NB肿瘤的基因表达谱，以阐明自发分化和回归的机制。计算分析确定了区分NB肿瘤和分化较差的NB肿瘤之间差异的基因。区分NB组包括与细胞成熟和凋亡相关的基因。与肿瘤发生有关的NB组分化差。未来，将添加考试；它认为我们希望能够发展新的治疗策略。