Mechanism of ischemia-reperfusion injury

缺血再灌注损伤机制

• 批准号: 12671756
• 负责人: UEDA Kouichi
• 金额: $ 2.05万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671756/
• 关键词: ischemia-reperfusion; macropharge; Dichloromethylene diphosphonate; rat skin flaps; 再灌流障害; 皮弁; 虚血

The purpose of this study is to evaluate whether macropharge is related to ischemia-reperfusion injury. The concern of macropharge was investigated by immuno-histochemical study in the ischemia-reperfusion model of rat skin flaps. The femoral artery and vein were clamped above and below the epigastric vessels for 11 h and then the clamp was released. Histological examination 12 h after reperfusion showed macropharge invasion into the flap and leukocyte invasion was recognized 24 h after reperfusion. Peroxidase activity in the flap presented a peak 48 h after reperfusion.Dichloromethylene diphosphonate (DMDP) was administered intravenously just before elevation of the right abdominal epigastric flap. However the effect by DMDP to ischemia-reperfusion injury was not recognized.

本研究的目的是探讨巨噬细胞是否与缺血再灌注损伤有关。在大鼠皮瓣缺血再灌注模型上，用免疫组织化学方法研究巨噬细胞的作用。将股动脉和股静脉夹在腹壁血管上方和下方11h后松开。再灌流后12h组织学检查显示巨噬细胞侵入皮瓣，再灌流后24h可见白细胞侵入。再灌流后48h，皮瓣内过氧化物酶活性达到高峰。在右腹上腹皮瓣抬高之前，静脉注射二氯亚甲基二磷酸(DMDP)。然而，DMDP对缺血再灌注损伤的作用尚未被认识到。

项目成果

上田 晃一: "皮フ・皮弁のreperfusion injuryと抗接着療法"Organ Biology. 8. 155-161 (2001)

Koichi Ueda：“皮瓣的再灌注损伤和抗粘连治疗”《器官生物学》8. 155-161 (2001)。

上田 晃一: "皮フ・皮弁のveperfusion injuryと抗接着療法"Organ Biology. 8. 155-161 (2001)

Koichi Ueda：“皮瓣的灌注损伤和抗粘连治疗”《器官生物学》8. 155-161 (2001)。