Effects of retinoic cid on adhesion molecules involving desmosome and hemidesmosome in gingival epithelial cells

维A酸对牙龈上皮细胞桥粒和半桥粒粘附分子的影响

• 批准号: 12671778
• 负责人: HATAKEYAMA Setsuko
• 金额: $ 1.66万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671778/
• 关键词: Gingival epithelium; desmosome; hemidesmosome; retinoic acid; desmoglein; desmocollin; elecron microscopy; cytokeratin; 超微形態; トランスジェニックマウス; E-カドヘリン; βカテニン; SV40ラージT抗原

Although retinoic acid was reported to compromise desmosome expression in the human epidermis through downregulation of desmosomal cadherins, it is unclear whether retinoic acid inhibits the formation of desmosomes and/or hemidesmosomes in oral epithelia. Whether retinoic acid disintegrated cell-cell and cell-matrix adhesions of oral epithelia was investigated with stratified gingival epithelial (GE1) cells. Ultrastructurally, retinoic acid-treatment (1 μM, 5-10 days) drastically reduced the number of desmosomes and completely destroyed hemidesmosomes. Keratinocyte phenotypes disappeared, accompanied by loss of tonofilaments. The transcripts of desmoglein 1 (ANOVA, P<0.01), desmocollin 1 (P<0.05), and keratin 13 (P<0.05) were significantly decreased, but expression of α6 and β4 subunits of integrin, components of hemidesmosomes, were not changed. These results indicated that retinoic acid also disintegrated desmosomes by depriving the cells of desmoglein 1 and desmocollin 1 in the oral mucosal epithelium. The decrease of keratin filaments, the intracellular cytoskeletal attachments to hemidesmosomes, was probably a cause of hemidesmosome loss.

尽管据报道视黄酸通过下调桥粒钙粘蛋白而损害人表皮中的桥粒表达，但尚不清楚视黄酸是否抑制口腔上皮中的桥粒和/或半桥粒的形成。用复层牙龈上皮（GE 1）细胞研究维甲酸是否能破坏口腔上皮细胞间及细胞间的粘附。在超微结构上，维甲酸处理（1 μM，5-10天）显著减少桥粒的数量，并完全破坏半桥粒。角质形成细胞表型消失，伴随着张力原丝的丢失。桥粒芯糖蛋白1（ANOVA，P<0.01）、桥粒胶原蛋白1（P<0.05）和角蛋白13（P<0.05）的转录水平显著降低，而半桥粒组成部分整合素α6和β4亚基的表达无明显变化。这些结果表明，视黄酸还通过剥夺口腔粘膜上皮细胞中的桥粒芯糖蛋白1和桥粒芯胶蛋白1来分解桥粒。细胞内附着于半桥粒的细胞骨架角蛋白丝的减少可能是半桥粒丢失的原因之一。

项目成果

畠山節子, 林秀一郎, 佐藤方信: "レチノイン酸によるマウス歯肉由来不死化上皮細胞株のデスモゾーム形成阻害"歯基礎誌. 43,妙録集. 145 (2001)

Settsuko Hatakeyama、Shuichiro Hayashi、Masanobu Sato：“视黄酸抑制小鼠牙龈来源的永生化上皮细胞系中的桥粒形成”Tetsuho Shisho 43，Myorokushu 145。

Hatakeyama, S., et al.: "Retinoic acid inhibits the formation of desmosome and hemidesmosome in multilayered oral mucosal epithelial cells"J Oral Pathol & Med. 31・5. 294 (2002)

Hatakeyama，S.等人：“视黄酸抑制多层口腔粘膜上皮细胞中桥粒和半桥粒的形成”J Oral Pathol & Med 31・5（2002）。

畠山節子, 林秀一郎, 佐藤方信: "レチノイン酸による不死化口腔上皮細胞の接着分子発現調節"第39回日本口腔組織培養学会プログラム・抄録集. 20 (2002)

Settsuko Hatakeyama、Shuichiro Hayashi、Masanobu Sato：“视黄酸对永生化口腔上皮细胞中粘附分子表达的调节”第 39 届日本口腔组织培养学会计划摘要集 20 (2002)。

Hatakeyama,S., et al.: "Establishment of gingival epithelial cell lines from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene."J Oral Pathol & Med.. (in press). (2001)

Hatakeyama,S. 等人：“从携带温度敏感猿病毒 40 大 T 抗原基因的转基因小鼠中建立牙龈上皮细胞系。”J Oral Pathol

Hatakeyama, S., et al.: "Retinoic acid inhibits the formation of desmosome in oral epithelial cells"J Dent Res (IADR abstracts). 80. 775 (2001)

Hatakeyama, S. 等人：“视黄酸抑制口腔上皮细胞中桥粒的形成”J Dent Res（IADR 摘要）。