MRI brain mapping image analysis of oral and maxillofacial region by various sensory stimuli

各种感觉刺激下口腔颌面部区域的MRI脑图图像分析

• 批准号: 12671825
• 负责人: KISHI Kanji
• 金额: $ 2.18万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671825/
• 关键词: functional MRI; gustatory stimulation; swallowing; fMRI; 脳機能画像

The aim of this study was to decide the adequate pulse sequence suitable for fMRI study of various sensory stimuli, and to establish the fixation of the head and image processing method, first of all. Secondly, we applied fMRI to the healthy volunteers on the tasks of gustatory stimulation and swallowing, and investigated the possibility of fMRI in the oral and maxillofacial region on clinical application.In 2000, first of all, we investigated the scanning method. We performed fMRI study of hand-grasping task by two pulse sequences, gradient echo type EPI(GE-EPI), and spin echo type EPI(SE-EPI), and compared the fMRI images by these two sequences. As a result, the specificity and accuracy of the signal detectability by SE-EPI were higher than those by GE-EPI. Therefore, we decided the SE-EPI sequence on the following study.The fixation of the head was performed by applying the hair-band and sponge to the head-coil and the head of the subjects. Visual stimulation and auditory stimulation were shut by using eye-mask and ear-plug.Image processing was performed by the software (Numaris) included in Magnetom Vision, and fMRI images were produced by the statistical calculation using z-score.Then, fMRI study by gustatory stimulation was performed. We used two tastants ; 1M NaCl and 3mM saccharin. These tastants were dropped into the oral cavity of the subjects through the tube, and fMRI scanning was done. As a result, pariental operculum, frontal operculum, and insula were mainly activated. There were no differences of activated areas between NaCl and saccharin.In 2001, fMRI by swallowing task was performed. Distilled water was injected into the oral cavity of the subjects at 3ml/sec. The subjects swallowed the water intermittently. As a result, primary motor cortex, and primary somatosensory cortex were mainly activated.

本研究的目的是确定适合于各种感觉刺激的fMRI研究的合适脉冲序列，并首先建立头部的固定和图像处理方法。其次，我们将fMRI应用于健康志愿者的味觉刺激和吞咽任务，并探讨了fMRI在口腔颌面部的临床应用的可能性。采用梯度回波型EPI(GE-EPI)和自旋回波型EPI(SE-EPI)两种脉冲序列对握手任务进行fMRI研究，并对两种序列的fMRI图像进行比较。结果表明，SE-EPI检测信号的特异性和准确性均高于GE-EPI。因此，我们在接下来的研究中确定了SE-EPI序列。通过将发带和海绵应用于受试者的头部线圈和头部来进行头部固定。用眼罩和耳塞关闭视觉刺激和听觉刺激，用Magnetom Vision自带的软件(Numaris)进行图像处理，并用z-SCORE进行统计计算生成fMRI图像，然后进行味觉刺激的fMRI研究。我们用了两种调味剂：1M氯化钠和3 mM糖精。通过试管将这些味精滴入受试者的口腔，并进行fMRI扫描。因此，顶盖、额盖和岛叶主要被激活。氯化钠和糖精的激活区域没有差异。2001年进行了吞咽任务的功能磁共振成像。将蒸馏水以3ml/秒的速度注入受试者的口腔。受试者断断续续地吞下水。结果，初级运动皮质和初级躯体感觉皮质主要被激活。