Immunocytochemical studies on DMBA induced lingual carcinoma in hamster

DMBA诱导仓鼠舌癌的免疫细胞化学研究

• 批准号: 60570932
• 负责人: KISHI Kanji
• 金额: $ 1.02万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570932/
• 关键词: Carcinoma; Immunocytochemistry; ケラチン蛋白

The experimental study was undertaken to examine the development of the distribution of intermediate-sized cytoskeltal filaments in the process of cancer formation and the radiation effect immunohistochemically. Supplementary to the experiments, clinical application were performed.Result 1. In normal and reacative stages, Cytokeratin (CK) was detectable in all of the squamous cell layers. Keratin-Squamous Epithelium-monoclonal (SE) and Keratin-Nonsquamous Epithelium-monoclonal (NSE) were observed in the superficial layer and Involucrin (Inv) positive reaction was seen in the superficial and the intermediate layers. But Vimentin (Vim) was negative in all layers.2. In the cases of the developed squamous cell carcinoma, keratin showed the mixed images of irragular and mosaic pattern of the antibody activity. CK staining became strong with the advancement of keratinization reflecting the squamous differentiation and a group fo cells showed the localized staining of CK at the periphery fo t … More he cytoplasm and nucleus. Inv stainig was positive in the differentiated area close to the outer layer of the keratinized portion showing a difinite tendency in the reacting area. Vim staning was positive throughout the undifferentiated area.3. In radiation group. There were no apparent differences in stainability between the rediated and the non-radiated groups.4. The experimental cytologic specimens SE was shown as diffuse positive reactive image in the entire tumor cytoplasm. But the localization of the staining could not be detected.5. In the cytologic specimens from clinicl malignant cases, tunor cells were strongly stained by SE in all cases and Vim was postitve in undifferenciated cases. The results showed that keratin was useful to decide the squamous origin and Vim as a marker for squamous differentiation. But there were no positive cases of Inv whihc might reflect a small number of cases in the experiment. Therefore, our study should be continued further to discuss this remained probem. Less

本实验研究了肿瘤形成过程中细胞间质微丝分布的演变及辐射效应。作为实验的补充，进行了临床应用。在正常和反应期，细胞角蛋白（CK）在所有鳞状细胞层中均可检测到。表皮浅层可见角蛋白-鳞状上皮-单克隆抗体（Keratin-Squamous epithelium-monoclonal，SE）和角蛋白-非鳞状上皮-单克隆抗体（keratin-nonsquamous epithelium-monoclonal，NSE），表皮浅层和中层可见Involucrin（Involucrin，Inv）阳性反应。波形蛋白（Vim）在各层均为阴性.在已发展的鳞状细胞癌中，角蛋白抗体活性呈不规则和镶嵌的混合图像。随着角化程度的加深，CK染色增强，反映了鳞状细胞的分化程度，有一组细胞在角化的周围出现了CK的局部染色。 ...更多信息 细胞质和细胞核。Inv染色在靠近角化部分外层的分化区呈阳性，在反应区有一定的趋势。Vim染色在整个未分化区均呈阳性.在放射组。辐射组与非辐射组之间无明显差异。实验性细胞学标本SE表现为弥漫性阳性反应图像，弥漫于整个肿瘤细胞质。但不能检测到染色的定位.在临床恶性肿瘤的细胞学标本中，肿瘤细胞均呈SE强染色，未分化肿瘤细胞呈Vim阳性。结果表明，角蛋白可作为判断鳞状细胞起源的指标，Vim可作为鳞状细胞分化的标志。但无Inv阳性病例，这可能反映了实验中的少数病例。因此，我们的研究应继续进一步讨论这一遗留问题。少

项目成果

HAYASE, Yasuhiro, WAKASA, Toru, TANAKA, Hideaki, HANADA, Kohji, KISHI, Kanji and MOTOI, Shin: "Application of Enzymed Labeled Antibody Method in Oral Cytology" The Journal of Japanese Society of Clinical Cytology (Okayama Branch). 6. 6-9 (1987)

HAYASE、Yasuhiro、WAKASA、Toru、TANAKA、Hideaki、HANADA、Kohji、KISHI、Kanji 和 MOTOI、Shin：“酶标记抗体方法在口腔细胞学中的应用”日本临床细胞学会杂志（冈山分会）。

早瀬康博,若狭亨,田中秀昌,花田孝二,岸幹二,元井信: 日本臨床細胞学会岡山支部会誌. 6. 6-9 (1987)

Yasuhiro Hayase、Toru Wakasa、Hidemasa Tanaka、Koji Hanada、Mikiji Kishi、Shin Motoi：日本临床细胞学会冈山分会杂志（1987 年）。