Immunocytochemical studies on DMBA induced lingual carcinoma in hamster

采用免疫组化方法对肿瘤形成过程中中等大小细胞骨架丝的分布及辐射效应进行了研究。在实验的基础上，进行了临床应用。结果1。在正常和反应期，细胞角蛋白（CK）在所有鳞状细胞层中均可检测到。浅层可见角蛋白-鳞状上皮单克隆（SE）和角蛋白-非鳞状上皮单克隆（NSE），浅层和中间层可见Involucrin （Inv）阳性反应。而Vimentin （Vim）在各层均呈阴性。在发展为鳞状细胞癌的病例中，角蛋白表现出不规则和马赛克模式的抗体活性混合图像。CK染色随着角化的进展而增强，反映了鳞状分化，一组细胞在胞浆和细胞核外周出现了CK的局部染色。角化部位外层附近的分化区呈阳性，反应区有一定的趋势。整个未分化区均为阳性。放射组。辐射组和未辐射组的染色性无明显差异。实验细胞学标本SE在整个肿瘤细胞质中呈弥漫性阳性反应象。但无法检测到染色的定位。在临床恶性病例的细胞学标本中，所有病例的肿瘤细胞都有强烈的SE染色，未分化病例的Vim阳性。结果表明，角蛋白可用于确定鳞状起源，Vim可作为鳞状分化的标志。但是没有出现阳性的Inv病例，这可能反映了实验中的少数病例。因此，我们的研究应继续深入探讨这一遗留问题。少

The experimental study was undertaken to examine the development of the distribution of intermediate-sized cytoskeltal filaments in the process of cancer formation and the radiation effect immunohistochemically. Supplementary to the experiments, clinical application were performed.Result 1. In normal and reacative stages, Cytokeratin (CK) was detectable in all of the squamous cell layers. Keratin-Squamous Epithelium-monoclonal (SE) and Keratin-Nonsquamous Epithelium-monoclonal (NSE) were observed in the superficial layer and Involucrin (Inv) positive reaction was seen in the superficial and the intermediate layers. But Vimentin (Vim) was negative in all layers.2. In the cases of the developed squamous cell carcinoma, keratin showed the mixed images of irragular and mosaic pattern of the antibody activity. CK staining became strong with the advancement of keratinization reflecting the squamous differentiation and a group fo cells showed the localized staining of CK at the periphery fo t … More he cytoplasm and nucleus. Inv stainig was positive in the differentiated area close to the outer layer of the keratinized portion showing a difinite tendency in the reacting area. Vim staning was positive throughout the undifferentiated area.3. In radiation group. There were no apparent differences in stainability between the rediated and the non-radiated groups.4. The experimental cytologic specimens SE was shown as diffuse positive reactive image in the entire tumor cytoplasm. But the localization of the staining could not be detected.5. In the cytologic specimens from clinicl malignant cases, tunor cells were strongly stained by SE in all cases and Vim was postitve in undifferenciated cases. The results showed that keratin was useful to decide the squamous origin and Vim as a marker for squamous differentiation. But there were no positive cases of Inv whihc might reflect a small number of cases in the experiment. Therefore, our study should be continued further to discuss this remained probem. Less