Serological and Histochemical Study of Tumor Markers on DMBA Induced Lingual Carcinoma

DMBA诱发舌癌肿瘤标志物的血清学和组织化学研究

• 批准号: 01480470
• 负责人: KISHI Kanji
• 金额: $ 3.71万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480470/
• 关键词: Tongue cancer; Tumor maker; Radiation; Histochemistry; Medication; Serology

There was a significant increase in the concentrations of putrescine, cadaverine and spermidine in the cancer tissue. The concehtrations of polyamines were decreased by X-radiation. There were no difference in the concentrations of polyamines in the cancer tissues after the administration of Cephalanthin as compared with that of the control. Therefore, the antitumor activity of Cephalanthin was not recognized. The decrease of the concentrations of polyamines in the tumor tissue by radiation effect was enhanced when combined with the administration of Cepharanthin. Induction HDC and ODC activity (Synthesizing enzymes of histamine and putrescine) was slight by the single administration of actinomycine D and by the painting with DMBA. But the induction of HDC activity was markedly increased with the combined application of DMBA and actinomycine D.TPA・BI antibody, was applied to immunohistochemistry to establish the method to detect the irradiation effect on DMBA-induced tongue squamous ce … More ll carcinomas in hamsters. Besides, the correlation between TPA・BI antibody and PKK-1, which were considered to be related to the carcinogenesis and the degree of the malignant progress was examined.Serum TPA values of the hamsters tended to increase in the group with carcinoma though the serum TPS values had no significant differences between the pre- and post- irradiation time.In the correlation between TPA・B1 antibody and PKK-1, biochemical analysis revealed that both TPA・B1 antibodies and PKK-1 reacted only with cytokeratin subunit No.18 (45kd) in the normal epitheliums and the squamous cell carcinomas of hamster tongues. Immunohistochemically, TPA・B1 antibodies were poitive at proliferating area of normal epitheliums and squamous cell carcinomas of hamster tongues, which was corresponded with the staining of PKK-1.In the cases of irradiated carcinomas, the cells with positive for TPA・B1 antibody were also positive of the anti-BrdU antibody, and the necrotic tissues were negative for both antibodies. Therefore, the area positive for TPA・B1 antibody was considered to be correlated with the proliferation of the tumor cells.As a result, TPA・B1 antibody, which has been used for the serological marker, was also considered to be useful to detect the histopathological changes of the tumor tissues caused by irradiation with single application of TPA・B1 antibody or combined with anti-BrdU antibody. Less

癌组织中腐胺、尸胺和亚精胺的浓度显著升高。X射线照射可降低多胺的含量。与对照组相比，给予头孢菌素后癌组织中多胺的浓度没有差异。因此，没有认识到Cephalanthin的抗肿瘤活性。与千金藤素联合应用时，肿瘤组织中多胺浓度的放射效应降低作用增强。单用放线菌素D和DMBA涂染诱导HDC和ODC活性（组胺和腐胺合成酶）轻微。应用TPA·BI抗体免疫组化方法，建立了DMBA诱导的舌鳞状细胞辐射效应的检测方法 ...更多信息 仓鼠的恶性肿瘤。此外，还研究了TPA·BI抗体与PKK-1之间的相关性，认为TPA·BI抗体与PKK-1之间的相关性与肿瘤的发生和恶性进展程度有关。生化分析表明，TPA·B1抗体和PKK-1抗体均只与金黄地鼠舌正常上皮和鳞状细胞癌中的细胞角蛋白亚基No.18（45 kd）反应。免疫组化结果显示，TPA·B1抗体在金黄地鼠舌正常上皮和鳞状细胞癌的增殖区呈阳性，与PKK-1的染色相一致。放射性癌中TPA·B1抗体阳性的细胞也呈抗BrdU抗体阳性，坏死组织两种抗体均为阴性。因此，TPA·B1抗体的阳性面积与肿瘤细胞的增殖有关，TPA·B1抗体作为血清学标志物，在单独应用TPA·B1抗体或与抗BrdU抗体联合应用时，也可用于检测肿瘤组织的放射性病理变化。少