Application of Laser Raman Micro-spectroscope for the Analysis of Resin-dentin Interdiffusion Zone

激光显微拉曼光谱仪在树脂-牙本质相互扩散区​​分析中的应用

• 批准号: 12671861
• 负责人: MIYAZAKI Masashi
• 金额: $ 1.09万
• 依托单位: Nihon-University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671861/
• 关键词: Hybrid Layer; Dentin Bonding; Raman Microscope; Resin penetration; Bonding Mechanism; 歯質接着; ラマン分光分析; レジンモノマー; 光重合型レジン; 重合率; 象牙質接着性; ボンディング材

The one of the most important mechanisms of dentin bonding is believed to be based on creation of resin-dentin interdiffusion (hybrid) layer. Since the width of this layer is quite thin, it has been very difficult to analyze and provide chemical composition of this layer. The purpose of this study was to investigate the method to analyze the resin-dentin interdiffusion zone by means of a high resolution Laser Raman microscopic system (System-2000, Renishaw). From the results of this study, the reliability of this system was determined as enough as to analyze a thin hybrid layer. The relative amounts of the hydroxyapatite of dentin (960 cm^<-1>, P-O totally stretching mode of phosphate), adhesive resin (640 cm^<-1>, aromatic ring stretch) and organic substances in adhesive resin and dentin (1450 cm^<-1>, scissoring vibration of the CH_2) were able to calculate by using Raman spectra detected from successive layer of resin-dentin interdiffusion zone. And also, it was found that the rate of residual double bonds was determined by comparing the vibration band of the methacrylate C=C bond at 1638 cm^<-1> with the C=O bond at 1717 cm^<-1>. It was concluded that using the Laser Raman microscopic observation enabled the precise analysis of the hybrid layer.

牙本质粘结的最重要机制之一被认为是基于树脂-牙本质相互扩散（混合）层的产生。由于该层的宽度非常薄，因此分析和提供该层的化学成分非常困难。本研究的目的是探讨利用高分辨率激光拉曼显微系统（System-2000，Renishaw）分析树脂-牙本质相互扩散区的方法。从这项研究的结果，该系统的可靠性被确定为足以分析一个薄的混合层。利用树脂-牙本质相互扩散区连续层的拉曼光谱，可以计算牙本质中羟基磷灰石（960 cm ~ 2<-1>，磷酸根的P-O全伸缩模式）、粘接树脂（640 cm ~ 2<-1>，芳香环伸缩模式）以及粘接树脂和牙本质中有机物（1450 cm ~ 2<-1>，CH_2的剪切振动模式）的相对含量。另外，通过比较甲基丙烯酸酯C=C键在1638 cm-1处的振动带与C=O键在1717 cm-1处的振动带，发现残留双键的比率<-1><-1>。结果表明，采用激光拉曼显微镜观察能够对杂化层进行精确分析。

项目成果

M Miyazaki, H Onose, BK Moore:: "Analysis of the dentin-resin interface by use of laser Raman spectroscopy"Dental Materials. 18(in printing). (2002)

M Miyazaki、H Onose、BK Moore：“使用激光拉曼光谱分析牙本质-树脂界面”牙科材料。

Masashi Miyazaki, Jun Higaki, Hirohiko Image, Seiichi Fijii, Hideo ONOSE, Hideki KAZAMA and Nobuhito IIDA: "Application of Laser Raman Micro-spectroscope for the Analysis of Resin-dentin Interdiffusion Zone"Adhesive Dentistry. 18. 43-50 (2000)

Masashi Miyazaki、Jun Higaki、Hirohiko Image、Seiichi Fijii、Hideo ONOSE、Hideki KAZAMA 和 Nobuhito IIDA：“激光拉曼显微光谱仪在树脂-牙本质相互扩散区​​分析中的应用”粘合牙科。

M Miyazaki, H Onose, N Iida, H Kazama: "Determination of residual double bonds in resin-dentin interface by laser Raman spectroscopy"Dental Material. (accepted). (2002)

M Miyazaki、H Onose、N Iida、H Kazama：“通过激光拉曼光谱测定树脂-牙本质界面中的残留双键”牙科材料。

M Miyazaki, H Onose, N Iida, H Kazama: "Determination of residual double bonds in resin-dentin interface by laser Raman spectroscopy"Dental Materials. (accepted).

M Miyazaki、H Onose、N Iida、H Kazama：“通过激光拉曼光谱测定树脂-牙本质界面中的残留双键”牙科材料。

宮崎真至,檜垣潤,稲毛寛彦,藤井清一,小野瀬英雄,風間秀樹,飯田伸仁: "レーザーラマン分光法を用いたレジン-象牙質接合界面のスペクトル分析に関する基礎的研究"接着歯学. 18. 43-50 (2000)

Masashi Miyazaki、Jun Higaki、Hirohiko Inage、Seiichi Fujii、Hideo Onose、Hideki Kazama、Nobuhito Iida：“使用激光拉曼光谱进行树脂-牙本质关节界面光谱分析的基础研究”粘合剂牙科。 18. 43-50 (2000)