Application of Laser Raman Micro-spectroscope for the Analysis of Resin-dentin Interdiffusion Zone

树脂-牙本质互扩散（杂化）层的形成是牙本质结合的重要机制之一。由于这一层的宽度很薄，分析和提供这一层的化学成分非常困难。本研究的目的是利用雷尼绍公司的高分辨率激光拉曼显微系统（system -2000, Renishaw）研究树脂-牙本质扩散区分析方法。根据研究结果，确定了该系统的可靠性，足以分析薄杂化层。利用连续层树脂-牙本质互扩散区拉曼光谱检测，可以计算出牙本质中羟基磷灰石（960 cm^<-1>， P-O完全磷酸盐拉伸模式）、粘接树脂（640 cm^<-1>，芳环拉伸模式）以及粘接树脂和牙本质中有机物（1450 cm^<-1>， CH_2剪切振动模式）的相对含量。通过比较甲基丙烯酸酯C=C键在1638 cm^<-1>处和C=O键在1717 cm^<-1>处的振动带，可以确定残余双键的速率。利用激光拉曼显微观察可以精确地分析杂化层。

The one of the most important mechanisms of dentin bonding is believed to be based on creation of resin-dentin interdiffusion (hybrid) layer. Since the width of this layer is quite thin, it has been very difficult to analyze and provide chemical composition of this layer. The purpose of this study was to investigate the method to analyze the resin-dentin interdiffusion zone by means of a high resolution Laser Raman microscopic system (System-2000, Renishaw). From the results of this study, the reliability of this system was determined as enough as to analyze a thin hybrid layer. The relative amounts of the hydroxyapatite of dentin (960 cm^<-1>, P-O totally stretching mode of phosphate), adhesive resin (640 cm^<-1>, aromatic ring stretch) and organic substances in adhesive resin and dentin (1450 cm^<-1>, scissoring vibration of the CH_2) were able to calculate by using Raman spectra detected from successive layer of resin-dentin interdiffusion zone. And also, it was found that the rate of residual double bonds was determined by comparing the vibration band of the methacrylate C=C bond at 1638 cm^<-1> with the C=O bond at 1717 cm^<-1>. It was concluded that using the Laser Raman microscopic observation enabled the precise analysis of the hybrid layer.