Development of multifunctional resins for robust dentin bonding

多功能牙本质粘接树脂的研制 项目摘要 目前的牙科修复体主要由于界面破坏/失效而遭受寿命缩短， 其导致微渗漏、敏感性、复发性龋齿、修复体移除/替换和广泛损失 牙齿结构健全。故障与当前的粘合系统失败有关， 形成持久的牙本质密封。目前的牙本质粘接策略主要依赖于微机械 渗透的树脂和脱矿牙本质层中暴露的胶原纤维之间的保留。强度 这种互锁或缠结取决于渗透树脂的质量和寿命， 混合层内的胶原原纤维。有大量证据表明，这一层的质量 是非常差的，并且微机械结合机制本质上是有问题的，不提供 在修复材料和牙本质之间形成牢固、紧密和持久的密封。体外和体内结果 包括我们在内的研究表明，以下关键问题阻碍了持久债券的形成 当使用当前的修复性粘结系统时。这些问题包括交互/绑定差/无交互/绑定 渗透树脂和胶原之间，渗透树脂质量差（由于单体/聚合物不足 转化和水解），以及酸蚀刻/未保护的胶原原纤维的降解。未受保护的 胶原蛋白被外源性细菌和内源性MMP（其被激活）降解 立即通过酸蚀刻），进行劣质树脂的水解。几乎不可能 获得牢固和持久的界面结合，而不会在化学和/或结合方面发生显著变化 战略布局显然，在革命性的化学反应之前，必须开发新的化学和新的键合概念。 可以实现牙齿矫正的改善。在本申请中，我们提出开发新颖的 因此，可以使用用于稳健、持久结合的单体来解决所有上述问题。这样的官能单体将是 设计为一端交联树脂，另一端交联胶原纤维，不仅稳定， 增加了树脂和胶原蛋白相的寿命，而且还在树脂和胶原蛋白相之间产生了紧密、牢固的结合， 树脂聚合物和牙本质胶原。这种方法是创新的，因为它代表了第一个系统的设计， 合理设计化学，同时解决所有三个关键挑战， 当前的粘合系统。该建议的总体假设是， 诱导胶原交联、强树脂-胶原相互作用和树脂交联将提供增强的 界面结构完整性和增加的耐久性在临床相关的牙本质基板的存在。 将使用组合方法/策略以及原位界面多尺度表征， 研究。这种方法将使我们能够确定最有前途的结构， 抗生物降解的修复树脂，其提供耐久的、结构上一体化的界面层， 临床相关牙本质基质。

Development of Multifunctional Resins for Robust Dentin Bonding Project Summary The current dental restorations suffer from reduced longevity mainly due to interfacial breakdown/failures, which cause microleakage, sensitivity, recurrent caries, restoration removal/replacement and extensive loss of sound tooth structure. The breakdown has been linked to the failure of current bonding systems to develop a durable seal to dentin. Current dentin bonding strategies mainly rely on micromechanical retention between infiltrated resin and exposed collagen fibrils in the demineralized dentin layer. Strength of this interlocking or entanglement depends on the quality and longevity of both the infiltrated resin and collagen fibrils within the hybrid layer. There is substantial evidence to suggest that the quality of this layer is very poor, and the micromechanical binding mechanism is intrinsically problematic, does not provide a strong, tight, and durable seal between restorative material and dentin. Results from both in vitro and in vivo studies including ours have indicated that the following critical issues inhibit the formation of a durable bond when using current restorative bonding systems. These issues include poor/no interactions/bindings between infiltrated resin and collagen, poor quality of infiltrated resin (due to inadequate monomer/polymer conversion and hydrolysis), and degradation of acid-etched/unprotected collagen fibrils. The unprotected collagen undergoes degradation by exogenous bacteria and endogenous MMPs (which are activated immediately by acid etching), proceeding with hydrolysis of poor quality resins. It is nearly impossible to obtain strong and durable interface bonding without dramatic alterations in chemistry and/or bonding strategies. Clearly new chemistry and new bonding concept must be developed before a revolutionary improvement in dental restorations can be accomplished. In this application, we propose to develop novel monomers for robust, durable binding to address all the above issues. Such functional monomers will be designed to crosslink resin at one end and crosslink collagen fibrils at the other, not only stabilizing and increasing the longevity of both resin and collagen phases but also creating a tight, strong bond between resin polymer and dentin collagen. The approach is innovative since it represents the first systematic design with rationally engineered chemistry to simultaneously tackle all the three critical challenges afflicting current bonding systems. The overall hypothesis of this proposal is that new restorative resins formulated to induce collagen crosslinking, strong resin-collagen interactions and resin crosslinking will provide enhanced interfacial structural integrity and increased durability in the presence of clinically relevant dentin substrates. A combinatory approach/strategy together with in situ interfacial multi-scale characterization will be used in the studies. This approach will allow us to identify the most promising structures for the development of a biodegradation-resistant restorative resin that provides a durable, structurally integrated interfacial layer with clinically relevant dentin substrates.