IMMUNOHISTOCHEMICAL STUDIES OF AGE-RELATED AND OSTEOARTHRITIC CHANGES IN TEMPOROMANDIBULAR JOINT

颞下颌关节年龄相关性和骨关节炎变化的免疫组织化学研究

• 批准号: 08672348
• 负责人: KAKUDO Kenji
• 金额: $ 1.34万
• 依托单位: OSAKA DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672348/
• 关键词: Temporomandibular joint; Senescence Accelerated Mouse; Human; Collagen; Glycosaminoglycan; Immunohistochemistry; Cndylar cortilage; II型コラーゲン; VI型コラーゲン; IX型コラーゲン; X型コラーゲン; XI型コラーゲン; I型コラーゲン; ヒアルロン酸結合蛋白

In this study, the following two aspects on the immunohistochemistry of the age-related and osteoarthric changes related with the temporomandibular joint of the Senescence Accelerated Mouse (SAM) and human were researched. In the condylar cartilage at 4 month-old SAMP8 compared with 2 month-old, the syaining of type I collagen decreased in the intermediate and hypertrophic zone. Type II collagen not only increased in the hypertrophic zone but also in the articular and intermediate zones. Type X collagen was present in the pericellular matrix of the hypertrophic zone, and increased stain intensity located diffusely in the intermediate zone. HABP was present throughout the cartilage, and decreased in the intermediate and hypertrophic zones. In the condylar cartilage of human, the stainingof type I collagen was present in the articular and intermediate zones. Type II collagen was present in the intermediate and hypertrophic zones. Type IV collagen was present in the articular and hypertrophic zones. Type X xollagen was present in the pericellular matrix of the hypertrophic zone. HABP was present throughout the articular and intermediate zones of cartilage and the pericellular matrix of the hypertrophic zone

本研究从以下两个方面对快速老化小鼠（SAM）和人类颞下颌关节的增龄性和骨关节相关性变化进行了免疫组化研究。与2月龄相比，4月龄SAMP8髁突软骨中，中间区和肥大区I型胶原的沉积减少。II型胶原不仅在肥大区增加，而且在关节和中间区。X型胶原存在于肥大区的细胞周围基质中，并且在中间区弥漫性染色强度增加。HABP存在于整个软骨，并在中间和肥大区减少。在人髁突软骨中，I型胶原主要分布于关节区和中间区。II型胶原蛋白存在于中间区和肥大区。IV型胶原蛋白存在于关节和肥大区。X型胶原存在于肥大区的细胞周基质中。HABP分布于软骨的关节区、中间区和肥大区的细胞周围基质

项目成果

Kakudo, K., Kurita, K., et al: "Newly Developed Arthroscopes with a channel for TMJ Sargery and its Clinical Application" Int.J,Oral Maxillofac.Sung.26(SP). 159 (1997)

Kakudo, K.、Kurita, K. 等人：“新开发的带有 TMJ Sargery 通道的关节镜及其临床应用”Int.J，Oral Maxillofac.Sung.26(SP)。

Koji YAMADA,Kenji KAKUDO,Takashi IKEO,Rikiya SHIRASU: "Immunohistochemical localization of type I,II,III,IV,VI and X collagen, CD44 and hyaluronic biding protein in temporomandibular joint of the Senescence Accelerated Mouse (SAM)" J Jpn Soc TMJ. 8. 361-3

Koji YAMADA、Kenji KAKUDO、Takashi IKEO、Rikiya SHIRASU：“衰老加速小鼠 (SAM) 颞下颌关节中 I、II、III、IV、VI 和 X 型胶原、CD44 和透明质酸结合蛋白的免疫组织化学定位” J Jpn Soc

安達 聡, 覚道 健治: "顎関節症患者における顎関節滑液ヒアルロン酸分子量ならびにN-アセチル-βグルコリミニダーゼ活性について" 日本顎関節学会雑誌. 9. 49-59 (1997)

Satoshi Adachi、Kenji Kakudo：“关于颞下颌关节紊乱患者颞下颌关节滑液中透明质酸的分子量和 N-乙酰基-β-葡萄糖苷酶活性”日本颞下颌关节学会杂志 9. 49-59（ 1997）

山田耕治, 覚道健治, 他: "ヒト顎関節におけるII,VI,IX,X,XI型コラーゲンの局在" 第11回日本顎関節学会総会(発表予定). (1998)

Koji Yamada、Kenji Kakudo 等人：“II、VI、IX 型胶原蛋白的定位，