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Research for Natural Anti-type I Allergic Compounds with Reactive Oxygen Species Scavenging Activity

具有活性氧清除活性的天然抗I型过敏化合物的研究

基本信息

批准号：
12672081
负责人：
ONO Masateru
金额：
$ 0.77万
依托单位：
Kyushu Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672081/
关键词：
Anti-type I Allergy Reactive Oxygen Species Natural Antioxidant ferric thiocyanate method antihyaluronidase activity flavonoid caffeoyl quinic acid tea polyphenol I型アレルギー DPPH Cammellia sinensis ポリフェノールアレルギーセドロンマティコハマゴウ /

项目摘要

Ninety-three compounds were isolated from the methanol or acetone extracts of six edible and medical plants, and their structures were elucidated on the basis of spectroscopic data as well as chemical evidence. Among these compounds, thirty phenolic compounds and forty-three polyphenols from tea leaves were examined for their antioxidative activity by the ferric thiocyanate method, and fifty-seven compounds showed a stronger antioxidative activity than α-tocopherol. Moreover, thirty-four antioxidants were assayed for their inhibitory effect on the activation of inactive hyaluronidase induced by compound 48/80. Twenty-one compounds indicated stronger inhibitory effect than tranilast. Especially, three compounds were identified as stronger inhibitors than disodium chromoglycate. The chemical structure and antihyaluronidase activity relationships suggested that catechol group seem to be important for the inhibitory effect. In addition, the inhibitory effect of flavonoid glycosides is less than those of the corresponding aglycone. In the case of caffeoyl quinic acid derivatives, those with free carboxyl group indicated a stronger inhibitory effect than methyl ester derivatives. Furthermore, proanthocyanidins which possess catechol group in the B-ring of upper unit showed a stronger inhibitory effect than those possess pyrogallol group in the B-ring of upper unit. Moreover, the acylation at the 3-OH in the lower unit of proanthocyanidin by galloyl group exhibited a higher inhibition. In the case of theasinensis, the inhibitory effect appeared to increase with the number of galloyl group attached to 3-OH position. From these results, flavonoids and caffeoyl quinic acid derivatives which possess catechol group and proanthocyanidins and theasinensins that possess galloyl group may be effective as an anti-type I allergic drug.

从6种食用和药用植物的甲醇或丙酮提取物中分离得到93个化合物，并根据光谱数据和化学证据对其结构进行了鉴定。用硫氰酸铁法测定了茶叶中30种酚类化合物和43种多酚类化合物的抗氧化活性，其中57种化合物的抗氧化活性强于α-生育酚。此外，研究了34种抗氧化剂对化合物48/80诱导的失活透明质酸酶活化的抑制作用。21种化合物的抑制作用强于曲尼司特。特别地，有三种化合物被鉴定为比铬糖酸二钠更强的抑制剂。化学结构和抗透明质酸酶活性的关系提示儿茶酚群可能对抑制作用起重要作用。此外，黄酮类苷的抑制作用小于相应的苷元。对于咖啡酰奎宁酸衍生物，游离羧基的抑制作用强于甲酯衍生物。含有邻苯二酚基团的原花青素比含有邻苯三酚基团的原花青素具有更强的抑制作用。此外，没食子酰基对原花青素较低单位3-OH的酰化表现出较高的抑制作用。在山核桃中，抑制效果似乎随着3-OH位置上的没食子酰基数量的增加而增加。综上所述，含有儿茶酚基和原花青素的类黄酮和咖啡酰奎宁酸衍生物以及含有没食子酰基的茶蛋白可能是一种有效的抗I型过敏药物。