Generation of ES cells that stably its translocation to mouse chromosome

为了研究额外拷贝的人类染色体对各种表型的基因剂量效应及其调控机制，我们将其易位到mChr的远端区域。10通过Cre/loxP介导的染色体易位和端粒指导的染色体截短的组合在胚胎干（ES）细胞和同源重组熟练的DT 40细胞中进行。例如，我们正在生产嵌合小鼠，这些小鼠保持来自ES细胞的DCR。这些小鼠应允许在各种组织中稳定维持和DCR（唐氏综合征关键区域）的稳定种系传递。此外，这些ES细胞将允许定位和鉴定负责各种表型的基因。

To investigate the gene dosage effects of an extra copy of human chromosome on various phenotypes and the regulatory mechanism, we translocated to the distal region of mChr. 10 by a combination of Cre/loxP mediated chromosome translocation and telomere directed chromosome truncation in embryonic stem (ES) cells and homologous recombination proficient DT40 cells. We are, for example, producing chimeric mice that maintain the DCR form the ES cells. These mice should allow stable maintenance in various tissue and stable germline transmission of the DCR (Down syndrome Critical Region). Furthermore, these ES cells will allow mapping and identification of the genes responsible for various phenotype.