グリオーマにおけるテロメレース活性およびテロメア長の臨床応用への検討
胶质瘤端粒酶活性和端粒长度检测的临床应用
基本信息
- 批准号:08457366
- 负责人:
- 金额:$ 3.01万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Telomeres progressively shorten with age in somatic cells in culture and in vivo because DNA replication results in the loss of chromosome ends. Normal cells in vivo and in vitro have a limited lifespan, whereas tumor cells can often grow indefinitely. Telomerase, a ribonucleoprotein which extends shortened chromosomal ends is re-expressed in most tumors. However, there are only a few reports on telomere length and telomerase in intracranial tumors. Thus, we examined telomere length and telomerase activity in gliomas, and FISH analysis of telomeres interphase nuclei for its usefulness as tumor markers. In this study, the telomere restriction fragments and telomerase activity were studied in 50 patients with various grades of gliomas. Telomerase activity was frequently positive in glioblastomas (86%), and telomere lengths were similar or shorter, compared with those in normal tissues. Some cases were repeatedly examined and had the positive telomerase activity during a tumor progression. In one case, however, tumor cells initially were negative for the activity and become positive, accompanied with a significant TRF shortening. Unlike most malignant tumors, longer TRFs than those in normal tissues were observed in 35% of the samples. Most of them were of astrocytoma (Grade 3). Fluorescence in situ hybridization showed that cases with longer telomerase-negative, and shorter (telomere-positive) telomeres can be detected, suggesting that FISH analysis in interphases and paraffin sections are useful for clinical applications.
体细胞的端粒随着年龄的增长而逐渐缩短,这是由于DNA复制导致染色体末端的丢失。体内和体外的正常细胞寿命有限,而肿瘤细胞通常可以无限生长。端粒酶是一种延长缩短的染色体末端的核糖核蛋白,在大多数肿瘤中重新表达。然而,关于端粒长度和端粒酶在颅内肿瘤中的作用的报道很少。因此,我们检测了胶质瘤中的端粒长度和端粒酶活性,并对端粒间期核进行了FISH分析,以确定其作为肿瘤标志物的用途。本研究对50例不同级别胶质瘤患者的端粒限制性片段和端粒酶活性进行了研究。端粒酶活性在胶质母细胞瘤中经常呈阳性(86%),端粒长度与正常组织相似或更短。一些病例在肿瘤进展过程中反复检查端粒酶活性呈阳性。然而,在一个病例中,肿瘤细胞最初的活性是阴性的,然后变成阳性的,伴随着显著的TRF缩短。与大多数恶性肿瘤不同,在35%的样本中观察到比正常组织更长的TRFs。多数为星形细胞瘤(3级)。荧光原位杂交显示,可以检测到端粒酶阴性较长和端粒阳性较短的病例,表明间期和石蜡切片的FISH分析具有临床应用价值。
项目成果
期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takahashi, C., Miyagawa, I., Kumano, S.et al.: "Detection of telomerase activity in porstate cancer by needlebiopsy." Euro.Urol.32. 494-498 (1997)
Takahashi, C.、Miyakawa, I.、Kumano, S.等人:“通过针吸活检检测前列腺癌中的端粒酶活性。”
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Takubo, K., Nakamura, K., Izumiyama, N.et al.: "Telomerase activity in esophageal carcinoma." J.Surg.Oncol.66. 88-92 (1997)
Takubo, K.、Nakamura, K.、Izumiyama, N.等人:“食管癌中的端粒酶活性。”
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- 影响因子:0
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Uejima,H., Shinohara,T., Nakayama,Y.et al.: "Mapping a novel oellylar senesoence gene to human chromosome 2q37 via irradiation microcell-mediated chromosome transfer" Mol.Carcinogen.(in press).
Uejima,H.、Shinohara,T.、Nakayama,Y.et al.:“通过辐照微细胞介导的染色体转移将新型 oellylar 衰老基因映射到人类染色体 2q37”Mol.Carcinogen。(出版中)。
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Tanaka,H.,Shimizu,M.,Horikawa,I.et al.: "Evidence for a putative telomerase repressor gene on the 3p14.2-p21.1 region" Genes,Chrom.& Cancer. in press.
Tanaka,H.、Shimizu,M.、Horikawa,I.et al.:“3p14.2-p21.1 区域上推定端粒酶阻遏基因的证据”Genes,Chrom。
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- 影响因子:0
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久郷裕之,押村光雄: "臨床染色体診断法" 金原出版社(古圧敏行監修), 5 (1996)
Hiroyuki Kugo、Mitsuo Oshimura:《临床染色体诊断方法》Kanehara Publishing(Toshiyuki Koo 监修),5(1996)
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- 影响因子:0
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OSHIMURA Mitsuo其他文献
OSHIMURA Mitsuo的其他文献
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{{ truncateString('OSHIMURA Mitsuo', 18)}}的其他基金
The elucidation of the carcinogenic mechanism of the Down's syndrome using chromosome engineering technology
利用染色体工程技术阐明唐氏综合症的致癌机制
- 批准号:
25221308 - 财政年份:2013
- 资助金额:
$ 3.01万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Gene therapy of Duchenne muscular dystrophy using own stem cells and human artificial chromosome
使用自身干细胞和人类人工染色体对杜氏肌营养不良症进行基因治疗
- 批准号:
21249022 - 财政年份:2009
- 资助金额:
$ 3.01万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Construction of human artificial chromosome for gene therapy of Duchenne muscular dystrophy
杜氏肌营养不良症基因治疗人类人工染色体的构建
- 批准号:
18390107 - 财政年份:2006
- 资助金额:
$ 3.01万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Genome-wide analysis of genomic impinting in cancer
癌症基因组印记的全基因组分析
- 批准号:
14026029 - 财政年份:2002
- 资助金额:
$ 3.01万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Generation of human chromosome-specific monoclonal antibodies using trans-chromosomic (TC) mice
使用转染色体 (TC) 小鼠生成人类染色体特异性单克隆抗体
- 批准号:
13357004 - 财政年份:2001
- 资助金额:
$ 3.01万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Generation of ES cells that stably its translocation to mouse chromosome
产生稳定易位至小鼠染色体的 ES 细胞
- 批准号:
12672200 - 财政年份:2000
- 资助金额:
$ 3.01万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Chromosomal mupping of a gene responsible for Bloom syndrome via microcell-mediated chromosome Transfer
通过微细胞介导的染色体转移对导致布卢姆综合征的基因进行染色体修饰
- 批准号:
04454539 - 财政年份:1992
- 资助金额:
$ 3.01万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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