Non-isotopic method for estimating erythrocyte mean age using erythrocyte creatine

使用红细胞肌酸估算红细胞平均年龄的非同位素方法

• 批准号: 12672245
• 负责人: OKUMIYA Toshika
• 金额: $ 1.98万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672245/
• 关键词: erythrocyte creatine; erythropoiesis; liver cirrhosis; splenomegaly; hypersplenism; cardiac valve; intravascular hemolysis; mechanical valve prosthesis; erythropoietin; renal anemia; hemodialysis

Erythrocyte creatine is a sensitive marker of erythrocyte age, and can be used to detect slight and continuous hemolysis. Excessive blood cell destruction caused by increased spleen function is important evidence of hypersplenism. We evaluated the usefulness of erythrocyte creatine as a marker of excessive erythrocyte destruction in patients with liver cirrhosis. The patients with splenomegaly showed significantly higher erythrocyte creatine than those without splenomegaly and healthy controls, but there was no significant difference in erythrocyte creatine between healthy controls and those without splenomegaly. Fourteen of the 15 patients with abnormally high erythrocyte creatine had splenomegaly. There were no significant differences in reticulocyte count between healthy controls and the patients with and without splenomegaly. Erythrocyte creatine showed good correlation with spleen size. Thus, erythrocyte creatine can be used for estimating hypersplenism in patients with liver cirr … More hosis.Cardiac vale replacement with mechanical prosthesis is associated with mild intravascular hemolysis. To estimate the intravascular hemolysis due to cardiac valve dysfunction, we analyzed erythrocyte creatine in 32 patients with prosthetic mitral and/or aortic valve, and the data were compared with cardiac valve flow dynamics. No significant differences in erythrocyte creatine and other hemolytic markers including serum lactic dehydrogenase, haptoglobin, indirect bilirubin and reticulocyte counts were observed between the patients in the presence and absence of regurgitations. Although erythrocyte creatine, serum lactic dehydrogenase and haptoglobin in the patients with prosthetic valve showed significant differences as compared to those in healthy controls, total peak transvalvular flow velocities (peak transmitral flow velocity + peak transaortic flow velocity) had significant correlation with only erythrocyte creatine. These results suggest that the intravascular hemolysis is attributed chiefly to transvalvular flow velocity rather than regurgitation in patients with mechanical valve prosthesis, and hence erythrocyte creatine is a possible marker for assessment of cardiac valve dysfunction. Less

红细胞肌酸是反映红细胞年龄的敏感指标，可用于检测轻度和持续性溶血。脾功能增强引起的血细胞过度破坏是脾功能亢进的重要证据。我们评估了红细胞肌酸作为肝硬化患者红细胞过度破坏的标志物的有用性。脾肿大组红细胞肌酸水平显著高于非脾肿大组和健康对照组，而健康对照组与非脾肿大组红细胞肌酸水平无显著性差异。15例红细胞肌酸异常增高的患者中有14例有脾肿大。网织红细胞计数在健康对照组与脾肿大组和非脾肿大组之间无显著差异。红细胞肌酸与脾脏大小呈良好的相关性。因此，红细胞肌酸可用于评估肝硬化患者的脾功能亢进， ...更多信息 机械瓣膜置换术与轻度血管内溶血有关。为评价心脏瓣膜功能障碍引起的血管内溶血，对32例二尖瓣和（或）主动脉瓣置换术患者的红细胞肌酸进行了测定，并与心脏瓣膜血流动力学进行了比较。红细胞肌酸和其他溶血标志物，包括血清乳酸脱氢酶，结合珠蛋白，间接胆红素和网织红细胞计数之间没有显着差异，观察患者的存在和不存在的过敏。尽管人工瓣膜患者的红细胞肌酸、血清乳酸脱氢酶和结合珠蛋白与健康对照组相比存在显著差异，但总跨瓣峰值流速（二尖瓣峰值流速+主动脉峰值流速）仅与红细胞肌酸显著相关。这些结果表明，血管内溶血的主要原因是跨瓣血流速度，而不是反流在机械瓣患者，因此，红细胞肌酸是一个可能的指标，以评估心脏瓣膜功能障碍。少

项目成果

Yoshiaki Takemoto, et al.: "Erythrocyte creatine as an index of the erythrocyte life span and erythropoiesis"Nephron. 86. 513-514 (2000)

Yoshiaki Takemoto 等人：“红细胞肌酸作为红细胞寿命和红细胞生成的指标”肾单位。

Yu-Fei Jiao, et al.: "Erythrocyte creatine as a marker of excessive erythrocyte destruction due to hypersplenism in patients with liver cirrhosis"Clinical Biochemistry. 34. 395-398 (2001)

Yu-Fei Jiao 等人：“红细胞肌酸作为肝硬化患者脾功能亢进导致红细胞过度破坏的标志物”《临床生物化学》。

Jiao Y-F, Okumiya T, Saibara T, Kudo Y, Sugiura T: "Erythrocyte creatine as a marker of excessive erythrocyte destruction due to hypersplenism in patients with liver cirrhosis"Clin Biochem. 34. 395-398 (2001)

Jiao Y-F、Okumiya T、Saibara T、Kudo Y、Sugiura T：“红细胞肌酸作为肝硬化患者脾功能亢进导致红细胞过度破坏的标志物”Clin Biochem。