Study of An Awareness Survey of Teachers About Genetically Modified Food and Teaching Materials

人们迫切地担心转基因作物的安全、标签和广泛商业使用所带来的环境风险。在我2000年的项目中，我对370名家庭经济专业的高中教师和研究生进行了一项关于转基因食品的认知调查。调查显示，他们对转基因食品的认识非常高，特别是在安全和标签方面。我们还调查了几家豆腐制造商可能存在的转基因大豆(抗除草剂大豆)的污染水平，方法是对喷洒除草剂后死亡的大豆进行筛选。对于五家豆腐生产商中的四家来说，转基因大豆的混合率似乎在25%至95%之间。在2001年的下一个项目中，我检查了可用于检测转基因大豆的商业试剂盒。我使用了一种试剂盒(Strategic Diagnotics Inc.，USA)，该试剂盒使用的是针对CP4EPSPS的抗体，CP4EPSPS是转基因大豆对除草剂的抗性产品。因此，就大豆的行叶和种子进行测试而言，该试剂盒是有价值的。但是，转基因大豆的抗原性在豆浆加热后被破坏，因此无法提供商品豆腐来检测转基因大豆。有必要研制另一种针对纯化的CP4EPSPS的多克隆抗体。对大豆脱脂叶片和种子的蛋白质进行了SDS-聚丙烯酰胺凝胶电泳法的分离，并在大豆叶片而不是大豆种子中检测到与46 kDa蛋白质相对应的特异性条带。我试图分离纯化这个蛋白，但未能完全纯化。应进一步进行CP4EPSPS的纯化和抗体的制备，为研制可视试剂盒作为学校转基因食品教材做好准备。

There are pressing concerns about safety, labeling and the environmental risks entailed in the wide commercial use of genetically modified (GM) crops. On my project in 2000, I conducted an awareness survey of GM foods on 370 high-school teachers of home economic subject with graduated student. The survey showed that their awareness for GM foods is extremely high, especially where safety and labeling are concerned. We also investigated the possible contamination levels of GM soybeans (soybeans resistant to herbicides) among several tofu makers, by screening dead soybeans after herbicide spraying. GM soybeans seem to be blended at rates of 25-95% for four of five tofu makers. On the next project in 2001, I examined the commercial kit to be available for the detection of GM soya. I used a kit (Strategic Diagnostic Inc., USA) which was used the antibody against CP4EPSPS, the product of GM soya of the resistance against herbicide. As a result , the kit was valuable as far as the row leaf and seed of soya was tested. But, the antigenisity of GM soya was destroyed after the heating of soya milk, therefore a commercial tofu was not available for materials of the detection of GM soya. It is necessary to develop another polyclonal antibody against purified CP4EPSPS. I examined the isolation of protein of the defatted leaf and seed of soya on SDS-polyacrylamide gel electrophoresis, and detected the specific band corresponding to 46kDa protein from soya leaf not soya seed. I tried to separate and purify this protein, but could not purify this protein completely. Further purification of CP4EPSPS and the production of antibody should be done for the development of visible kit as a teaching materials of GM food at school.