Structure and function of atherogenic human apolipoprotein E7 variant

致动脉粥样硬化人类载脂蛋白E7变体的结构和功能

• 批准号: 02670397
• 负责人: MAEDA Hideo
• 金额: $ 1.22万
• 依托单位: Naruto University of Education
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670397/
• 关键词: lipoprotein; atherosclerosis; hyperlipidemia; very low density; apolipoprotein E; Zucker rat; obesity

Two oligopeptides corresponding to the residue 238-252 of apolipoprotein E3(Ag 3 : GluVal-Arg-Ala-Lys-Leu-Glu-Glu-Gln-Ala-Gln-Gln-Ile-Arg-Leu)and two substituted residues 238252(Ag 7 : Glu-Val-Arg-Ala-Lys-Leu-Lys-Lys-Gln-Ala-Gln-Gln-Ile-Arg-Leu)were synthesized. These oligopeptides of Ag 3 and Ag 7 were coupled with bovine serum albumin and injectedinto rabbit to produce antiserum. Both anti Ag 3 antibody and anti Ag 7 antibody did not show any cross-reaction each other and didn't discriminate each antigen.Another study was undertaken to characterize the difference in liver cytosol proteins between lean and obese Zucker rats. It was shown that unidentified 120 kDa protein which exist in liver cytosol fraction, but not seen in mitochondorial fraction was increased to several folds in genetically obese Zucker rats. But, this 120 kDa protein was induced by refeeding of high carbohydrate diet overnight followed after fasting for 48 hr of lean Zucker rats. One of the characterization of 120 kDa protein is similar to the receptor of apolipoprotein E reported by other investigator. These observations strongly suggested that 120 kDa protein seen in liver cytosol fraction may be responsible for lipogenesis in obese Zucker rats.

合成了载脂蛋白E3残基238-252对应的两个寡肽（Ag 3: gluval - arg - ala - lys - leu - glu - gln - ala - gln - gln - ile - arg - leu）和两个取代残基238252（Ag 7: gluval - arg - ala - lys - leu - lys - lys - gln - ala - gln - gln - ile - arg - leu）。将银3和银7寡肽与牛血清白蛋白偶联，注射到家兔体内制备抗血清。抗ag3抗体和抗ag7抗体相互间不存在交叉反应，且不区分各抗原。另一项研究进行了表征瘦和肥胖的Zucker大鼠之间肝细胞质蛋白的差异。结果表明，在遗传肥胖的Zucker大鼠中，存在于肝细胞质部分而不存在于线粒体部分的未知的120kda蛋白增加了数倍。但是，这种120 kDa的蛋白质是在瘦Zucker大鼠禁食48小时后再饲喂高碳水化合物饮食过夜诱导的。120kda蛋白的一个特征与其他研究者报道的载脂蛋白E受体相似。这些观察结果强烈提示，肥胖Zucker大鼠肝细胞质中120 kDa蛋白可能与脂肪生成有关。

项目成果

Hideo Maeda: "Increased Unidetified 120kDa protein in liver Cytosol of Genetically Obese Zucker Rats"

Hideo Maeda：“遗传性肥胖 Zucker 大鼠肝脏细胞质中未鉴定的 120kDa 蛋白质增加”

Hideo Maeda: "Increased Unidentified 120 kDa Protein in Liver Cytosol of Genetically Obese Rats"

Hideo Maeda：“遗传性肥胖大鼠肝细胞溶质中未知的 120 kDa 蛋白质增加”

Hideo Maeda: "Increased Unidentified 120 kDa Protein in Liver Cytosol of Genetically Obese Rats."

Hideo Maeda：“遗传性肥胖大鼠肝细胞溶质中未知的 120 kDa 蛋白质增加。”