Study on environmental pollution with mutagens in high mortality areas by lung cancer

采用高效液相色谱法测定了30个表层土壤样品中1，3-二硝基芘（DNP）、1，6-DNP和1，8-DNP的含量。在所有检查的土壤样品中均检测到DNP异构体，其在土壤样品中的浓度范围为几皮克/克土壤。3-硝基苯蒽酮（NBA）是一种主要的诱变剂，在土壤样品中检测到，表现出强烈的致突变性。为了研究3-NBA在环境中的分布，我们建立了3-NBA在大气颗粒物和表层土壤中的分析方法。3-通过HPLC部分纯化NBA并通过具有荧光检测器HPLC定量。近畿地区环境空气中的3-NBA浓度为几皮克/立方米，近畿、东海和关东地区土壤样本中的浓度为几纳克至几百纳克/克土壤。

1,3-Dinitropyrene (DNP), 1,6-DNP and 1,8-DNP in 30 surface soil samples were quantified by high performance liquid chromatography equipped with a platinum/rhodium catalyst column and a fluorescence detector. DNP isomers were detected in all soil samples examined, and their concentration in soil samples ranged from a few pg/g of soil. 3-Nitrobenzanthrone (NBA) was detected as a major mutagen in a soil sample that showed strong mutagenicity. To clarify the distribution of 3-NBA in the environment, we developed an analytical method of 3-NBA in airborne particles and surface soil. 3-NBA was partially purify by HPLC and quantified by HPLC with a fluorescence detector. Concentrations of 3-NBA in ambient air in the Kinki area were a few pg/m3, and those in soil samples from the Kinki, Tokai and Kanto areas ranged from a few ng to a few hundred ng/g of soil.