Detection and genotoxicity of nitroarenes in ambient air and surface soil

环境空气和表土中硝基芳烃的检测和遗传毒性

• 批准号: 16590098
• 负责人: WATANABE Tetsushi
• 金额: $ 1.6万
• 依托单位: Kyoto Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590098/
• 关键词: nitroarene; air; soil; genotoxicity; mutagen

Organic extracts from surface soil samples collected in Osaka and Aichi prefectures showed potent mutagenicity in S.typhimurium TA98 in the absence of S9 mix, inducing 1,240 - 233,000 revertants/mg of organic extract. Each soil extract was separated by chromatography using a Sephadex LH-20 column and a silica gel column. The fractions with potent mutagenicity were subsequently separated into five fractions, Fr. 1 - Fr. 5, using an Ultra pack ODS column. The contribution ratios of these five fractions to the total mutagenicity of each soil extract were similar ; Fr. 1 : 45% ; Fr. 2 : 15% ; Fr. 3 : 25% ; Fr. 4 : 5 ; Fr. 5 : 10%. These five fractions were further separated by HPLC. In consequence, it was found that mutagenic potencies of Fr. 1 and Fr. 3 were attributed to dinitropyrene (DNP) and 3,6-dinitrobenzo[e]pyrene (DNBeP), respectively. Major mutagens in Fr. 2, Fr. 4, and Fr. 5 were isolated from each soil extract, and these mutagens were expected to be nitroarenes on the basis of their mass spectra. Retention times of these mutagens in HPLC were not consistent with those of nitrosrenes, which had been detected in the environment. 3,6-DNBeP showed extremely potent mutagenicity in TA98 without S9 mix, inducing 285,000 revertants/nmol. This potency was comparable to that of 1,8-DNP, which is the strongest mutagen reported to date. Because 3,6-DNBeP showed higher mutagenicity in YG1024 than TA98, 3,6-DNBeP was thought to be activated by O-acetyltranferase for mutagenicity.

在不存在S9混合物的情况下，从大坂和爱知县采集的表层土壤样品的有机提取物对鼠伤寒沙门氏菌TA 98显示出强致突变性，诱导1，240 - 233，000个回复突变体/mg有机提取物。每种土壤提取物通过使用Sephadex LH-20柱和硅胶柱的色谱分离。随后使用Ultra pack ODS色谱柱将具有强致突变性的组分分离为5个组分，Fr.1-Fr.5。这五个组分对每种土壤提取物总致突变性的贡献率相似; Fr. 1：45% ; Fr. 2：15% ; Fr. 3：25% ; Fr. 4：5 ; Fr. 5：10%。通过HPLC进一步分离这五个级分。结果发现，Fr.1和Fr.3的致突变性分别归因于二硝基苯并[e]芘（DNP）和3，6-二硝基苯并[e]芘（DNBeP）。从每种土壤提取物中分离出Fr.2、Fr.4和Fr.5中的主要诱变剂，根据其质谱，预计这些诱变剂为硝基芳烃。这些诱变剂在HPLC中的保留时间与在环境中检测到的亚硝胺的保留时间不一致。3，6-DNBeP在不含S9混合物的TA 98中显示出极强的致突变性，诱导285，000个回复突变体/nmol。该效力与1，8-DNP相当，1，8-DNP是迄今为止报道的最强诱变剂。由于3，6-DNBeP在YG 1024中的致突变性高于TA 98，因此认为3，6-DNBeP的致突变性是由O-乙酰转移酶激活的。

项目成果

Detection of a Novel Mutagen, 3,6-Dinitrobenzo[e]pyrene, as a Major Contaminant in Surface Soil in Osaka and Aichi Prefectures, Japan

在日本大阪和爱知县表层土壤中检测到一种新型诱变剂 3,6-二硝基苯并[e]芘作为主要污染物

• 发表时间: 2005
• 期刊: Chemical Research in Toxicology 18
• 作者: K.Sugihara;S.Kitamura;T.Yamada;T.Okayama;S.Ohta;Tetsushi Watanabe
• 通讯作者: Tetsushi Watanabe

Mutagenicity of Surface Soils in Urban Areas of Aichi Prefecture, Japan, and Bangkok, Thailand

日本爱知县和泰国曼谷城市地区表层土壤的致突变性

• 发表时间: 2005
• 期刊: Journal of Health Science 51(6)
• 作者: Hashimoto Y;Kaneko Y;Tsukamoto E;Frankowski H;Kouyama K;Kita Y;Niikura T;Aiso S;Bredesen DE;Matsuoka M;Nishimoto I;Kitamura et al.分担(R.C.Gupta編集);Tetsushi Watanabe
• 通讯作者: Tetsushi Watanabe