STUDIES ON THE EFFECTS OF DIESEL EXHAUSTUDIES PARTICLES ON HUMAN HEALTH

柴油机尾气颗粒对人体健康影响的研究

• 批准号: 12680551
• 负责人: MORI Yoki
• 金额: $ 1.6万
• 依托单位: HEALTH SCIENCES UNIVERSITY OF HOKKAIDO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680551/
• 关键词: diesel exhaust particles; estrogenic activity; anti-estrogenic activity

Diesel cars exhaust 30-100 times more particles (DEP) then gasoline engine cars. DEP is one of the most important air pollution substances, especially, in the large city. It was known that soluble organic fraction of DEP contains thousands of organic compound. Previously, the authors found that DEP could react with o-oxygen to produce superoxide anion radical in vitro, and that extract of DEP could cause disease of respiratory organ by production of reactive oxygen intermediates (ROI) and inhibition of degradation of ROI. Further, we found that buffer extract of DEP could activate the alternative pathway of human complement system.Recently, it is reported that many compounds in environment possess the estrogenic activity. Estrogenic compounds in environment could modulate the endocrine systems and reproductions of animals and human.In this project, we aimed the establishment of the methods for systematic isolation of compounds in DEP, and evaluation of estrogenic and anti-estrogenic activities of DEP. DEP were extracted serially, with hexane, benzene, dichloromethane, methanol and 1M ammonia. About 80 % of substances in DEP were extracted in this methods. Anti-estrogenic activity was found in extracts of benzene, dichloromethane, and methanol, and estrogenic activity was not found in these extracts. Hexane extract was fractionated to acidic, phenolic and neutral fractions, and it was proved that neutral fraction of hexane extract containes estrogenic compounds. It was found that heterologous compounds in DEP extracts possess the estrogenic activity and one of which was found to be 4, 6-dimethydibenzothiophene. Estrogenic and anti-estorogenic compounds in DEP could affect the endocrine system and reproduction of human.

柴油车排放的颗粒物(DEP)是汽油发动机车的30-100倍。DEP是最重要的大气污染物质之一，尤其是在大城市中。众所周知，DEP中的可溶性有机部分含有数千种有机化合物。以前，作者发现DEP在体外可以与氧反应产生超氧阴离子自由基，DEP提取物通过产生活性氧中间产物(ROI)和抑制ROI的降解而导致呼吸器官疾病。此外，我们还发现DEP的缓冲提取物可以激活人类补体系统的替代途径。最近，有报道称，环境中的许多化合物都具有雌激素活性。环境中的雌激素化合物可以调节动物和人类的内分泌系统和生殖系统，本课题旨在建立DEP中化合物的系统分离方法，并对DEP的雌激素和抗雌激素活性进行评价。DEP用正己烷、苯、二氯甲烷、甲醇和1M氨水依次提取。DEP中约80%的物质是用这种方法提取的。苯、二氯甲烷、甲醇提取物具有抗雌激素活性，未发现雌激素活性。将正己烷提取物分为酸性组分、酚性组分和中性组分，证明正己烷萃取物的中性组分中含有雌激素类化合物。结果表明，DEP提取物中的异源化合物具有雌激素样活性，其中4，6-二甲基二苯并噻吩类化合物为DEP提取物中的一种。DEP中的雌激素和抗雌激素化合物可影响人体的内分泌系统和生殖功能。

项目成果

Yoshino, S., Hayashi, H., Taneda.S., Mori, Y.et al.: "Effect of diesel exhaust particles on induction of oral tolerance in mice"Toxicological Sciences. 66. 293-297 (2002)

Yoshino, S.、Hayashi, H.、Taneda.S.、Mori, Y.等人：“柴油机尾气颗粒对小鼠口服耐受诱导的影响”毒理学科学。

Yoshlno S., Hayashi H., Taneda S., Takano H., Sagal M. and Mori Y.: "Effect of diesel exhaust particles on induction of oral tolerance in mice"Toxicological Sciences. 66 (3). 293-297 (2002)

Yoshlno S.、Hayashi H.、Taneda S.、Takano H.、Sagal M. 和 Mori Y.：“柴油机尾气颗粒对小鼠口服耐受诱导的影响”毒理学科学。

Taneda, S., HayashiH., Mori, Y, Sekl, K.et al.: "Estrogenic and anti-estrogenic activities of two types of diesel exhaust particles"Toxicology. 170(1). 153-161 (2002)

Taneda, S.、HayashiH.、Mori, Y、Sekl, K.等人：“两种柴油机尾气颗粒的雌激素和抗雌激素活性”毒理学。

Yoshino, S., Hayashi, H., Taneda, S., Mori, Y.et al.: "Estrogenic and anti-estrogenic activities of acidic, phenolic and neutral fractions of hexane extract of diesel exhaust particles"Environmental Sciences. (in press).

Yoshino, S.、Hayashi, H.、Taneda, S.、Mori, Y.等人：“柴油机尾气颗粒己烷提取物的酸性、酚类和中性馏分的雌激素和抗雌激素活性”环境科学。

Taneda, S., Hayashi H., Seki, K., Mori Y.et al.: "Estrogenic and anti-estrogenic activities of acidic, phenolic and neutral fractions of hexane extract of diesel exhaust particles"Environmental Sciences. (in press).

Taneda, S.、Hayashi H.、Seki, K.、Mori Y.等人：“柴油机尾气颗粒己烷提取物的酸性、酚类和中性馏分的雌激素和抗雌激素活性”环境科学。