Study on Design of Bioelastic and Chemotactic Matrix for Artificial Brood Vessel

在2000年至2002年，基于具有自组装和弹性性质的重复五肽序列Vat-Pro-Gly-Val-Gly（VPGVG）的聚合物，基于具有细胞迁移性质的重复六肽序列Val-Gly-Val-Ala-Pro-Gly（VGVAPG）的聚合物，两种无规共聚物，（高VGVAPG含量：hp 10和低VGVAPG含量：hp 50），基于上述VPGVG和VGVAPG序列和五种无规共聚物（较高的Gly-Arg-Gly-Asp-Ser（GRGDS）含量：pp 500和较低的GRGDS含量：pp 20）和具有细胞粘附和抗血栓性质的GRGDS序列。测试了hp 10和hp 50的趋化活性，结果hp 10比hp 50更有活性。测试了pp 20 -500的细胞粘附活性，结果，pp 500比pp 20更有活性。2003年合成了两种VGVAPG含量高于hp 10和hp 50的无规共聚物（hp 100和hp 500），并测试了它们的自组装能力和趋化活性。结果表明，与hp 10和hp 50相比，hp 100和hp 500都没有表现出更高的自组装和趋化能力。人工血管生物弹性和趋化性基质的设计正在进一步研究中。

In 2000 to 2002, a polymer based on the repeating pentapeptide sequence Vat-Pro-Gly-Val-Gly(VPGVG) which possesses the property of self-assembly and elasticity, a polymer based on the repeating hexapeptide sequence Val-Gly-Val-Ala-Pro-Gly(VGVAPG) which possesses the property of cell migration, two random copolymers(a high VGVAPG content : hp10 and a low VGVAPG content : hp50) based on VPGVG and VGVAPG sequences described above and five random copolymers(a higher Gly-Arg-Gly-Asp-Ser(GRGDS) content : pp500 and a less GRGDS content : pp20) based on VPGVG sequence described above and GRGDS sequence which possesses the property of cell adhesion and anti-thrombus were synthesized. The chemotactic activity of hp10 and hp50 was tested and as results, hp10 was more active than hp50. The cell adhesive activity of pp20-500 was tested and as results, pp500 was more active than pp20. In 2003, two random copolymers(hp 100 and hp500) in which both copolymers are higher contents of VGVAPG than hp10 and hp50 were synthesized and tested their abilities for self assembly and chemotactic activity. As results, compared with hp10 and hp50, both hp100 and hp500 did not exhibited higher abilities of self-assembly and chemotaxis. Further studies on design of bioelastic and chemotactic matrix for artificial brood vessel is under investigation.