Study on Design of Bioelastic and Chemotactic Matrix for Artificial Brood Vessel

人工育雏容器生物弹性趋化基质设计研究

• 批准号: 12680837
• 负责人: OKAMOTO Kouji
• 金额: $ 2.37万
• 依托单位: Kyushu Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680837/
• 关键词: Elastin-derived peptide; Self-assembly; Elasticity; Cell migration; Cell adhesion; Anti-thrombus; 抗血栓性; 弾性線維; 細胞呼び寄せ型; 弾性マトリックス; 人工血管

In 2000 to 2002, a polymer based on the repeating pentapeptide sequence Vat-Pro-Gly-Val-Gly(VPGVG) which possesses the property of self-assembly and elasticity, a polymer based on the repeating hexapeptide sequence Val-Gly-Val-Ala-Pro-Gly(VGVAPG) which possesses the property of cell migration, two random copolymers(a high VGVAPG content : hp10 and a low VGVAPG content : hp50) based on VPGVG and VGVAPG sequences described above and five random copolymers(a higher Gly-Arg-Gly-Asp-Ser(GRGDS) content : pp500 and a less GRGDS content : pp20) based on VPGVG sequence described above and GRGDS sequence which possesses the property of cell adhesion and anti-thrombus were synthesized. The chemotactic activity of hp10 and hp50 was tested and as results, hp10 was more active than hp50. The cell adhesive activity of pp20-500 was tested and as results, pp500 was more active than pp20. In 2003, two random copolymers(hp 100 and hp500) in which both copolymers are higher contents of VGVAPG than hp10 and hp50 were synthesized and tested their abilities for self assembly and chemotactic activity. As results, compared with hp10 and hp50, both hp100 and hp500 did not exhibited higher abilities of self-assembly and chemotaxis. Further studies on design of bioelastic and chemotactic matrix for artificial brood vessel is under investigation.

2000年至2002年，基于具有自组装和弹性的重复五肽序列VAT-Pro-Gly-Val-Gly(VPGVG)的聚合物，基于重复六肽序列Val-Gly-Val-Ala-Pro-Gly(VGVAPG)的具有细胞迁移特性的聚合物，基于上述VPGVG和VGVAPG序列合成了两种随机共聚物(高VGVAPG含量：HP10和低VGVAPG含量：HP50)和五种基于上述VPGVG序列和具有细胞黏附和抗血栓特性的GRGDS序列的随机共聚物(较高Gly-Arg-Gly-Asp-Ser(GRGDS)含量：PP500和较少GRGGDS含量：PP20)。测定了HP10和HP50的趋化活性，结果表明，HP10的趋化活性高于HP50。对pp20-500的细胞黏附活性进行了测定，结果表明，pp500的细胞黏附活性高于pp20。2003年，合成了两种VGVAPG含量高于HP10和HP50的无规共聚物(HP100和HP500)，并测试了它们的自组装能力和趋化活性。结果表明，与HP10和HP50相比，HP100和HP500没有表现出更强的自组装和趋化能力。人工育种器的生物弹性和趋化基质的设计正在进一步研究中。

项目成果

岡元 孝二: "弾性線維の構造と機能"日本皮膚科学会誌. 112. 1726-1728 (2002)

Koji Okamoto：“弹性纤维的结构和功能”日本皮肤病学会杂志 112. 1726-1728 (2002)。

岡元孝二: "Interaction of Solution and Solid States of Elastin-derived Peptides with Contractile and Synthetic Phenotypes of Vascular Smooth Muscle Cells"Peptide Science 2001. 123-126 (2002)

Koji Okamoto：“弹性蛋白衍生肽的溶液和固态与血管平滑肌细胞的收缩和合成表型的相互作用”肽科学 2001. 123-126 (2002)

K.Kaibara, K.Okamoto, K.Miyakawa: "Characterization of Proteinic Coacervate Formation : From Primeval Cell Model to Biofunctionality Materials"Handbook of Polyelectrolytes and Their Applications, Volume 3: Applications of Polyelectrolytes and Theoretical

K.Kaibara、K.Okamoto、K.Miyakawa：“蛋白质凝聚层形成的表征：从原始细胞模型到生物功能材料”聚电解质及其应用手册，第 3 卷：聚电解质的应用和理论

Y.Fukumoto: "Elastin-derived Polypeptides,(VPGVG)n,(VPGV)n, and (VPG)n : Coacervation Property and Specific Interaction with Metal Ions"Peptide Science 2000. 189-192 (2001)

Y.Fukumoto：“弹性蛋白衍生的多肽，(VPGVG)n，(VPGV)n，和(VPG)n：凝聚特性和与金属离子的特异性相互作用”肽科学2000。189-192(2001)

M.Brones, I.Maeda, S.Kamisato, N.Takami, K.Okamoto: "Chemotactic Activation of Macrophages by Elastin Hexapeptide Sequences and Its Signaling Mechanism"Peptide Science 1999. 221-227 (2000)

M.Brones、I.Maeda、S.Kamisato、N.Takami、K.Okamoto：“弹性蛋白六肽序列对巨噬细胞的趋化激活及其信号机制”肽科学 1999. 221-227 (2000)