Study on Biomaterials for Drug Delivery Sysytem Based on Dynamic Structural Change of Molecular Assembly of Elastin Peptides

基于弹性蛋白肽分子组装动态结构变化的药物递送系统生物材料研究

• 批准号: 07680946
• 负责人: OKAMOTO Kouji
• 金额: $ 1.41万
• 依托单位: Kyushu Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680946/
• 关键词: Elastin; Molecular assembly; Dynamic structural change; Drug delivery system

<1995>A high polymer based on the repeating pentapeptide sequence Val-Pro-Gly-Val-Gly which has the property of salf-assembly, a peptide Arg-Gly-Tyr-Ser-Leu-Gly which can be phosphorylated by protein kinase, and two peptides Gly-Ser-Asn-His-Gly and Gly-Arg-Asn-Thr-Gly in which Asn residue can be converted to Asp residue, were synthesized. Arg-Gly-Tyr-Ser-Leu-Gly was phosphorylated by cyclic AMP dpendent protein kinase. The half-lives for Asn-Asp conversion were 6 days and 28 days for Gly-Ser-Asn-His-Gly and Gly-Arg-Asn-Thr-Gly.<1996>Poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser-Leu-Gly)] which consists of the indicated molar ratios was synthesized. This polymer was phosphorylated by cyclic AMP dependent protein kinase for 24 hr at 30ﾟC.The resulting phosphorylated polymer poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser (PO_4^<2->) -Leu-Gly)] was studied for self-assembly. The temperature for the inverse temperature transition (Tm) of the phosphorylated polymer was shifted to higher temperature giving a shift of 14ﾟC.The phosphorylated polymer was incubated with alkaline phosphatase for 4 hr at 30ﾟC to yield a dephosphorylated poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser-Leu-Gly)]. Tm was shifted to lower temperature giving a shift of 13ﾟC.This result indicated complete dephosphorylation of phosphorylated polymer.

<1995>合成了具有自组装特性的Val-Pro-Gly-Val-Gly五肽高分子、可被蛋白激酶磷酸化的Arg-Gly-Tyr-Ser-Leu-Gly五肽、可将Asn残基转化为Asp残基的Gly-Ser-Asn-His-Gly和Gly-Arg-Asn-Thr-Gly五肽。Arg-Gly-Tyr-Ser-Leu-Gly被环腺苷酸依赖性蛋白激酶磷酸化。Gly-Ser-Asn-His-Gly和Gly-Arg-Asn-Thr-Gly的Asn-Asp转化半衰期分别为6天和28天。<1996>合成了由指定摩尔比组成的聚[15（Val-Pro-Gly-Val-Gly），（Arg-Gly-Tyr-Ser-Leu-Gly）]。该聚合物在30 ℃下被环AMP依赖性蛋白激酶磷酸化24小时。研究了所得磷酸化聚合物poly [15（Val-Pro-Gly-Val-Gly），（Arg-Gly-Tyr-Ser（PO_4^<2->）-Leu-Gly）]的自组装。将磷酸化聚合物的逆温度转变（Tm）的温度移动到更高的温度，得到14 ℃的移动。将磷酸化聚合物与碱性磷酸酶在30 ℃下孵育4小时，得到脱磷酸化的聚[15（Val-Pro-Gly-Val-Gly），（Arg-Gly-Tyr-Ser-Leu-Gly）]。Tm向较低温度移动，产生13 ℃的移动。该结果表明磷酸化聚合物完全去磷酸化。

项目成果

神里早月: "Characteristic interaction of Ca^<2+> ions with elastin coacervate : ion transport study across coacervate layers of α-elastin and elastin model polypeptide,(Val-Pro-Gly-Val-Gly)n" Biopolymers. 39. 189-198 (1996)

Hayazuki Kamisato：“Ca ^ 2+ 离子与弹性蛋白凝聚层的特征相互作用：跨 α-弹性蛋白和弹性蛋白模型多肽（Val-Pro-Gly-Val-Gly）n 凝聚层的离子传输研究”生物聚合物 39。 189-198 (1996)

上村祐子: "Elastin peptides induce monocyte chemotaxis by increasing the level of cyclic GMP,an intracellular second messenger" Peptides : Chemistry,Structure & Biology. (印刷中). (1996)

Yuko Uemura：“弹性蛋白肽通过增加细胞内第二信使环 GMP 的水平来诱导单核细胞趋化性”肽：化学、结构与生物学（1996 年出版）。

甲斐原 梢: "Elastin peptide coacervate as a model of extracellular elastomeric matrix : jonic transport study" Peptide Chemistry 1996. 493-496 (1997)

Kozue Kaihara：“弹性蛋白肽凝聚层作为细胞外弹性体基质的模型：jonic 运输研究”肽化学 1996. 493-496 (1997)

輪干浩史: "Stimulation of cell proliferation and autoregulation of elastin expression by elastin peptide VPGVG in cultured chick vascular smooth muscle cells" FEBS Letters. 368. 215-219 (1995)

Hiroshi Washo：“在培养的鸡血管平滑肌细胞中通过弹性蛋白肽 VPGVG 刺激细胞增殖和弹性蛋白表达的自动调节”FEBS Letters 368. 215-219 (1995)。

上村祐子: "Elastin peptides induce monocyte chemotaxis by increasing the level of cyclic GMP,an intracellular second messenger" Peptides : Chemistry,Structure and Biology. 412-413 (1996)

Yuko Uemura：“弹性蛋白肽通过增加细胞内第二信使环 GMP 的水平来诱导单核细胞趋化性”肽：化学、结构和生物学 412-413 (1996)。