Chemiluminescence method of ruthenium complex in non-aqueous solvent

非水溶剂中钌配合物的化学发光方法

• 批准号: 13640611
• 负责人: YAMAZAKI Shigeo
• 金额: $ 0.32万
• 依托单位: Okayama University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640611/
• 关键词: ruthenium complex; chemiluminescence; tris (bipyridyl) ruthenium (III); background; aprotonic solvent; PF_6^-; acitive-oxygen generator; thiuram; tris(bipyridyl)ruthenum(III); PF_6^-; 非水溶液; 化学発光

Improving the sensitivity and the applicability of chemiluminescence method using ruthenium complexIt was concermed that the sensitivity of chemiluminescence method using ruthenium complex depended the background strength, which should be depressed in order to improve the sensitivity of the chemiluminescence method. Since tervalent tris (bipyridyl) ruthenium (III) is reducted by water with chemiluminescence which cause the increasing of background, we proposed that the use of non-aqueous aprotonic solvent for chemiluminescence reagent causes the decreasing of the background chemiluminescence and the improving the sensitivityPreparation method of this (bipyridyl) ruthenum (III) hexafuluorophosphate was developed in gram order scale. The investigation using acetonitrile solution of tris (bipyridyl) ruthenum (III) hexafuluorophosphate as a chemiluminescence reagent was performed. The results described below were found; a) Acetonitril, which is aprotonic solvent, can dissove tris (bipyridyl) ruthenum (III) hexafuluorophoshate, b) The tervalent ruthenium complex is reduced by water with chemiluminescence which couse the mcreasing of background, c) Employing acetonitrile as a solvent for chemiluminescence reagent, the background chemiluminescence is decreased and sensitivity is improved, d) The tervalent ruthenium complex is reduced by water and the reducing reaction decrease the life-time of chemiluminescence reagent, e) Employing acetonitrile as a solvent for chemiluminescence reagent the life-time of chemiluminescence reagent is increased to over 7 days, g) Acetonitrile solution of tris (bipyridyl) ruthenum (III) hexafuluorophosphate is useable to detection of aromatic compound, h) The chemiluminescence mechanism is based on generation of active oxygen from aromatic compound by irradiation of UV-light, I) Aliphatic compounds containing peroxide moiety release active oxygen by irradiation of UV-light

提高钌络合物化学发光法的灵敏度和适用性钌络合物化学发光法的灵敏度取决于背景强度，为了提高其灵敏度，应降低背景强度。由于三价联吡啶钌（Ⅲ）被水还原产生化学发光，导致背景发光增加，我们提出用非水质子溶剂作化学发光试剂，降低了背景发光，提高了灵敏度，并在克级规模上建立了该（联吡啶）钌六氟磷酸盐的制备方法。研究了以三（联吡啶）六氟磷酸钠（Ⅲ）的乙腈溶液为化学发光试剂。发现了下述结果; a）乙腈是非质子溶剂，可溶解三羟甲基氨基甲烷。（联吡啶）六氟磷酸二铵（Ⅲ），B）水还原钌配合物，使其产生化学发光，使背景发光增加，c）以乙腈为溶剂，降低了背景发光，提高了灵敏度，d）三价钌配合物被水还原，还原反应降低了化学发光试剂的寿命。e）使用乙腈作为化学发光试剂的溶剂，化学发光试剂的寿命提高到7天以上，g）tris的乙腈溶液（联吡啶）六氟磷酸二氢铵（III）可用于检测芳族化合物，h）化学发光机理基于通过UV光照射从芳族化合物产生活性氧，I）含有过氧化物部分的脂肪族化合物通过紫外光照射释放活性氧

项目成果

N.Niina: "Detection of Hydroperoxide by Chemicalluminescence"Chromatography. 22. 17-18 (2001)

N.Niina：“化学发光检测氢过氧化物”色谱法。