Chemiluminescence method of ruthenium complex in non-aqueous solvent

提高钌配合物化学发光方法的灵敏度和适用性认为，钌配合物化学发光方法的灵敏度依赖于背景强度，为了提高化学发光方法的灵敏度，应降低背景强度。由于三价（联吡啶基）钌（III）被水还原产生化学发光，导致背景发光增加，我们提出使用非水非质子溶剂作为化学发光试剂，使背景化学发光降低，灵敏度提高，并以克为量级发展了该（联吡啶基）六氟磷酸钌（III）的制备方法。以三（联吡啶）六氟磷酸钌（III）乙腈溶液为化学发光试剂进行了研究。结果如下所述：a)乙腈为非质子溶剂，可溶解三（联吡啶）钌（III）六氟膦酸盐；b)三价钌配合物被水还原，产生化学发光，使背景发光增加；c)乙腈为化学发光试剂溶剂，降低了背景化学发光，提高了灵敏度。d)三价钌配合物被水还原，还原反应降低了化学发光试剂的寿命，e)采用乙腈作为化学发光试剂的溶剂，化学发光试剂的寿命增加到7天以上，g)三（联吡啶）六氟磷酸钌（III）乙腈溶液可用于检测芳香族化合物。h)化学发光机理是基于芳香族化合物在紫外线照射下生成活性氧，I)含过氧化物的脂肪族化合物在紫外线照射下释放活性氧

Improving the sensitivity and the applicability of chemiluminescence method using ruthenium complexIt was concermed that the sensitivity of chemiluminescence method using ruthenium complex depended the background strength, which should be depressed in order to improve the sensitivity of the chemiluminescence method. Since tervalent tris (bipyridyl) ruthenium (III) is reducted by water with chemiluminescence which cause the increasing of background, we proposed that the use of non-aqueous aprotonic solvent for chemiluminescence reagent causes the decreasing of the background chemiluminescence and the improving the sensitivityPreparation method of this (bipyridyl) ruthenum (III) hexafuluorophosphate was developed in gram order scale. The investigation using acetonitrile solution of tris (bipyridyl) ruthenum (III) hexafuluorophosphate as a chemiluminescence reagent was performed. The results described below were found; a) Acetonitril, which is aprotonic solvent, can dissove tris (bipyridyl) ruthenum (III) hexafuluorophoshate, b) The tervalent ruthenium complex is reduced by water with chemiluminescence which couse the mcreasing of background, c) Employing acetonitrile as a solvent for chemiluminescence reagent, the background chemiluminescence is decreased and sensitivity is improved, d) The tervalent ruthenium complex is reduced by water and the reducing reaction decrease the life-time of chemiluminescence reagent, e) Employing acetonitrile as a solvent for chemiluminescence reagent the life-time of chemiluminescence reagent is increased to over 7 days, g) Acetonitrile solution of tris (bipyridyl) ruthenum (III) hexafuluorophosphate is useable to detection of aromatic compound, h) The chemiluminescence mechanism is based on generation of active oxygen from aromatic compound by irradiation of UV-light, I) Aliphatic compounds containing peroxide moiety release active oxygen by irradiation of UV-light