Chemiluminescence method of ruthenium complex in non-aqueous solvent

提高化学发光法的灵敏度和适用性研究认为，化学发光法的灵敏度依赖于背景强度，为了提高化学发光法的灵敏度，应降低本底强度。由于三价三(联吡啶基)Ru(III)在水中被化学发光还原，导致背景发光增加，我们提出使用非水非质子溶剂作为化学发光剂会导致背景发光的降低，并提高了灵敏度。以六氟代磷酸三(联吡啶)Ruthenum(III)的乙腈溶液作为化学发光剂进行了研究。发现了以下描述的结果；A)乙腈是一种非质子溶剂，能溶解三(联吡啶基)六氟磷酸根的化学发光试剂；b)用化学发光的水还原三价Ru的络合物，使本底发光增加；c)用乙腈作为化学发光剂的溶剂，降低了本底发光强度，提高了灵敏度；d)水还原了三价Ru的络合物，还原反应缩短了化学发光剂的使用寿命；e)以乙腈为化学发光剂的溶剂，化学发光剂的寿命延长到7天以上；g)六氟磷酸三(联吡啶)的乙腈溶液可用于芳香族化合物的检测，H)化学发光机理是基于紫外光照射下芳香族化合物产生活性氧，i)含有过氧基团的脂肪族化合物在紫外光照射下释放出活性氧

Improving the sensitivity and the applicability of chemiluminescence method using ruthenium complexIt was concermed that the sensitivity of chemiluminescence method using ruthenium complex depended the background strength, which should be depressed in order to improve the sensitivity of the chemiluminescence method. Since tervalent tris (bipyridyl) ruthenium (III) is reducted by water with chemiluminescence which cause the increasing of background, we proposed that the use of non-aqueous aprotonic solvent for chemiluminescence reagent causes the decreasing of the background chemiluminescence and the improving the sensitivityPreparation method of this (bipyridyl) ruthenum (III) hexafuluorophosphate was developed in gram order scale. The investigation using acetonitrile solution of tris (bipyridyl) ruthenum (III) hexafuluorophosphate as a chemiluminescence reagent was performed. The results described below were found; a) Acetonitril, which is aprotonic solvent, can dissove tris (bipyridyl) ruthenum (III) hexafuluorophoshate, b) The tervalent ruthenium complex is reduced by water with chemiluminescence which couse the mcreasing of background, c) Employing acetonitrile as a solvent for chemiluminescence reagent, the background chemiluminescence is decreased and sensitivity is improved, d) The tervalent ruthenium complex is reduced by water and the reducing reaction decrease the life-time of chemiluminescence reagent, e) Employing acetonitrile as a solvent for chemiluminescence reagent the life-time of chemiluminescence reagent is increased to over 7 days, g) Acetonitrile solution of tris (bipyridyl) ruthenum (III) hexafuluorophosphate is useable to detection of aromatic compound, h) The chemiluminescence mechanism is based on generation of active oxygen from aromatic compound by irradiation of UV-light, I) Aliphatic compounds containing peroxide moiety release active oxygen by irradiation of UV-light