Understanding of gene regulation in halophilic Archaea: Characterization of the transcriptome, DNA protein interactions, and posttranslational control

了解嗜盐古细菌的基因调控：转录组表征、DNA 蛋白质相互作用和翻译后控制

• 批准号: 5316374
• 负责人: Professor Dr. Jörg Soppa
• 金额: --
• 依托单位: Arbeitskreis Biologie und Genetik von Prokaryonten
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5316374?language=en
• 关键词: Understanding; gene; regulation; halophilic; Archaea

Informational proteins of Archaea in e.g. replication, transcription and translation, are homologous to eukaryotic proteins. In contrast, many metabolic proteins of Archaea are closer related to bacterial proteins. The regulation of "bacterial-like" genes in organisms with an "eukaryotic-like" transcriptional apparatus is not understood. Halobacterium salinarum is an excellent model system to study archaeal gene regulation, because it is metabolically very versatile and can grow aerobically, microaerobically, phototrophically, fermentatively and by anaerobic respiration. It is able to perceive a variety of chemical and physical environmental signals. Furthermore, the genome sequence is available, functional genomic studies have been initiated and molecular and biochemical techniques are far developed. In the proposed project DNA microarrays will be used to characterize differential expression of all genes of wildtype cells and of specifically constructed mutants in response to different environmental changes and to cell density-generated signals. This will allow to identify regulatory hierachies and circuits. The binding sites of selected gene-specific and of global regulators will be characterized in vitro and in vivo. The interaction of selected regulatory proteins with basal transcription factors and with DNA will be quantitavely characterized on the molecular level.

在复制、转录和翻译等方面，真核生物的信息蛋白与真核生物蛋白同源。相比之下，许多代谢蛋白质的葡萄球菌是密切相关的细菌蛋白。在具有“真核生物样”转录装置的生物体中，“细菌样”基因的调控尚不清楚。盐生盐杆菌是研究古细菌基因调控的理想模式系统，因为它在代谢上非常灵活，可以有氧、微氧、光养、发酵和无氧呼吸生长。它能够感知各种化学和物理环境信号。此外，基因组序列是可用的，功能基因组研究已经开始，分子和生物化学技术也得到了长足的发展。在拟议的项目中，DNA微阵列将用于表征野生型细胞和特定构建的突变体的所有基因的差异表达，以响应不同的环境变化和细胞密度产生的信号。这将允许识别监管层级和电路。将在体外和体内表征所选基因特异性和全局调节剂的结合位点。所选的调控蛋白与基础转录因子和DNA的相互作用将在分子水平上定量表征。