Intermolecular Gene Conversion in halophilic Archaea.

嗜盐古菌中的分子间基因转换。

• 批准号: 427230939
• 负责人: Professor Dr. Jörg Soppa
• 金额: --
• 依托单位: Arbeitskreis Biologie und Genetik von Prokaryonten
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/427230939?language=en
• 关键词: Intermolecular; Gene; Conversion; halophilic; Archaea.

Gene conversion is defined as the non-reciprocal transfer of information from one DNA sequence to a homologous (but not identical) sequence. Gene conversion occurs in all three domains of life and also in viruses. Gene conversion is involved in many different processes, from DNA repair at stalled replication forks to the generation of genetic diversity in meiosis. Gene conversion has mostly been studied in eukaryotes, and the studies with bacteria concentrated on intramolecular gene conversion, which results in concerted evolution of gene families or in antigenic variation. Intermolecular gene conversion between different genome copies of polyploid prokaryotes has hardly been studied. It can lead to the equalization of genome copies and represents an escape from Muller’s ratchet (a theory predicting that polyploid prokaryotes should not be able to exist). In the proposed project the haloarchaeon Haloferax volcanii will be used to study various aspects of intermolecular gene conversion for the equalization of genome copies. An experimental approach has been established that enables the quantitative analysis of unselected gene conversion events. The following objectives will be pursuit: 1) Characteristic features of unselected gene conversion will be analyzed, e.g. the influence of the extent of genome differences on gene conversion efficiencies; 2) The influence of external and environmental parameters on intermolecular gene conversion will be analyzed: 3) Proteins will be identified, which are involved in or essential for intermolecular gene conversion; and 4) Intermolecular gene conversion between genes of different species will be analyzed. Together, these approaches will lead to an unprecedented insight into intermolecular gene conversion for the equalization of genome copies in polyploid prokaryotes.

基因转换被定义为信息从一个DNA序列到同源（但不相同）序列的非相互转移。基因转换发生在生命的所有三个领域，也发生在病毒中。基因转换涉及许多不同的过程，从停滞复制叉的DNA修复到减数分裂中遗传多样性的产生。基因转换主要在真核生物中进行研究，细菌的研究集中在分子内基因转换，这导致基因家族的协同进化或抗原变异。多倍体原核生物不同基因组拷贝之间的分子间基因转换几乎没有研究。它可以导致基因组拷贝的均等化，并代表了对穆勒棘轮的逃避（一种预测多倍体原核生物不应该存在的理论）。在拟议的项目中，Haloferax volcanii将用于研究基因组拷贝均衡的分子间基因转换的各个方面。已经建立了一种实验方法，使得能够定量分析β-内酰胺酶基因转换事件。本研究的主要目的是：1）分析转基因的特征，如基因组差异程度对转基因效率的影响; 2）分析外部和环境参数对分子间转基因的影响; 3）鉴定分子间转基因所涉及或必需的蛋白质;和4）分析不同物种的基因之间的分子间基因转换。总之，这些方法将导致一个前所未有的洞察分子间基因转换的基因组拷贝在多倍体原核生物的均衡。