Near-field Scanning Optical Microscopy and Nano-electrophoresis

近场扫描光学显微镜和纳米电泳

• 批准号: 13650867
• 负责人: HORIE Kazuyuki
• 金额: $ 2.11万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13650867/
• 关键词: Nanooptics; Near-field scanning optical microscope; NSOM; Nanoelectrophoresis; Polystyrene gel; Latex; Refractive index control; Fluorescence control; 近接場光学顕微鏡; 電気泳動; ポリマー; ゲル; 微粒子; ポリスチレン; 蛍光プローブ

In this research we constructed a near-field scanning optical microscope (NSOM) which can be applied to study electrophoresis in nano space, and aimed at the separation analysis of fluorescent-labeled polymer particles and biomaterials and monitoring of material transfer on nanometer scale resolution. Fluorescent images of polystyrene latex moving in a specially-prepared liquid electrophoresis cell with 3 mm width, 25 mm length, and 150 μm depth were observed on the NSOM stage. Brownian motion of the polystyrene latex loosely interacting with the poly-N-isopropylacrylamide network was noticed. Fluorescent images of a single core-shell type microparticle as well as photochemically-induced refractive index and fluorescence patterning were also studied with this NSOM system.

本研究构建了一种可应用于纳米空间电泳研究的近场扫描光学显微镜（NSOM），旨在实现荧光标记聚合物颗粒和生物材料的分离分析以及纳米尺度上的物质转移监测。在NSOM台上观察了在专门制备的宽3 mm、长25 mm、深150 μm的液体电泳池中移动的聚苯乙烯乳胶的荧光图像。聚苯乙烯胶乳与聚-N-异丙基丙烯酰胺网络之间的相互作用为布朗运动。利用该系统还研究了单个核壳型微粒的荧光图像以及光化学诱导的折射率和荧光图案化。

项目成果

K.Horie, S.Yamada, S.Machida, S.Takahashi, Y.Isono, H.Kawaguchi: "Dansyl Fluorescence and Local Structure of Dansyl-labeled Core-shell and Core-hair Type Microspheres in Solution"Macromol.Chem.Phys.. 204・1. 131-138 (2003)

K.Horie、S.Yamada、S.Machida、S.Takahashi、Y.Isono、H.Kawaguchi：“溶液中丹磺酰标记的核-壳和核-毛型微球的丹磺酰荧光和局部结构”Macromol.Chem。物理.. 204・1. 131-138 (2003)

Y.Kato, H.Muta, S.Takahashi, K.Horie, T.Nagai: "Large Photoinduced Refractive Index Change of Polymer Films Containing and Bearing Norbornadiene Groups and its Application of Submicron-scale Refractive-index Patterning"Polym. J.. 33・11. 868-873 (2001)

Y.Kato、H.Muta、S.Takahashi、K.Horie、T.Nagai：“含降冰片二烯基团的聚合物薄膜的大光致折射率变化及其在亚微米级折射率图案化中的应用” .33・11.868-873 (2001)

M. Kobayashi, S. Machida, S. Takahashi, K. Horie, H. Yoshikawa, H. Masuhara: "Synthesis and Single Particle Spectroscopy of Novel Fluorescent Dendrimers with Highly Efficient Energy Harvesting"Chem. Lett.. 2002(3). 394-395

M. Kobayashi、S. Machida、S. Takahashi、K. Horie、H. Yoshikawa、H. Masuhara：“具有高效能量收集的新型荧光树枝状聚合物的合成和单粒子光谱”Chem。

S.Takahashi, H.Muta, S.Machida, K.Horie: "Modification of Surface Morphology and Refractive Index of a Polymer Film Using Nearfield Scanning Optical Microscopy"Trans. Mater. Res. Soc. Jpn.. 26・3. 953-956 (2001)

S.Takahashi、H.Muta、S.Machida、K.Horie：“使用近场扫描光学显微镜改变聚合物薄膜的表面形态和折射率”，Res. 26・3。 -956 (2001)

K. Horie, S. Yamada, S. Machida, S. Takahashi, Y. Isono, H. Kawaguchi: "Dansyl Fluorescence and Local Structure of Dansyl-labeled Core-shell and Core-hair Type Microspheres in Solution"Macromol. Chem. Phys.. 204(1). 131-138 (2003)

K. Horie、S. Yamada、S. Machida、S. Takahashi、Y. Isono、H. Kawaguchi：“溶液中丹磺酰标记的核-壳和核-毛型微球的丹磺酰荧光和局部结构”Macromol。