Near-field Scanning Optical Microscopy and Nano-electrophoresis

本研究构建了一种可应用于纳米空间电泳研究的近场扫描光学显微镜（NSOM），旨在实现荧光标记聚合物颗粒和生物材料的分离分析以及纳米尺度上的物质转移监测。在NSOM平台上观察了聚苯乙烯胶乳在宽3 mm、长25 mm、深150 μm的液体电泳池中运动的荧光图像。聚苯乙烯胶乳与聚-N-异丙基丙烯酰胺网络之间的相互作用为布朗运动。利用该系统还研究了单个核壳型微粒的荧光图像以及光化学诱导的折射率和荧光图案化。

In this research we constructed a near-field scanning optical microscope (NSOM) which can be applied to study electrophoresis in nano space, and aimed at the separation analysis of fluorescent-labeled polymer particles and biomaterials and monitoring of material transfer on nanometer scale resolution. Fluorescent images of polystyrene latex moving in a specially-prepared liquid electrophoresis cell with 3 mm width, 25 mm length, and 150 μm depth were observed on the NSOM stage. Brownian motion of the polystyrene latex loosely interacting with the poly-N-isopropylacrylamide network was noticed. Fluorescent images of a single core-shell type microparticle as well as photochemically-induced refractive index and fluorescence patterning were also studied with this NSOM system.