Molecular basis on the regulation of methylation that controls transposition of the plant transposons

控制植物转座子转座的甲基化调节的分子基础

• 批准号: 13660001
• 负责人: KISHIMA Yuji
• 金额: $ 2.56万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660001/
• 关键词: Antirrhinum majus; transposon; Tam3; low temperature stress; DNA methylation; gel shift assay; 5-aza-cytidine; トランスポゾンTam3; 低温依存性転移; DNAのメチル化; 重亜硫酸ナトリウム処理実験; タンパク質のDNA結合実験; 転移酵素タンパク質

The transposition frequency of Tam3 in Antirrhinum, unlike that of most other cut-and-paste type transposons, is tightly controlled by temperature : Tam3 transposes rarely at 25℃, but much more frequently at 15℃. Here we studied the mechanism of the low-temperature-dependent transposition (LTDT) of Tam3. Our results strongly suggest that LTDT is not likely to be due to either transcriptional regulation or posttranscriptional regulation of the Tam3 Tpase gene. We found that temperature shift induced a remarkable change of the methylation state unique to Tam3 sequences in the genome : higher temperature resulted in hypermethylation, while lower temperature resulted in reduced methylation. The methylation state was reversible within a single generation in response to a temperature shift. Although our data demonstrate a close link between LTDT and the methylation of Tam3, they also suggest that secondary factor(s) other than DNA methylation is involved in repression of Tam3 transposition.

与大多数其他剪切粘贴型转座子不同，金鱼草中Tam3的转座频率受到温度的严格控制：Tam3在25℃下很少转座，但在15℃下转座频率更高。在这里，我们研究了低温依赖性转座（LTDT）的Tam3的机制。我们的研究结果强烈表明，LTDT是不太可能是由于转录调控或转录后调节的Tam3 Tpase基因。我们发现，温度变化诱导了基因组中Tam3序列特有的甲基化状态的显着变化：较高的温度导致超甲基化，而较低的温度导致甲基化降低。甲基化状态是可逆的，在一个单一的代内响应于温度变化。虽然我们的数据表明LTDT和Tam3甲基化之间存在密切联系，但它们也表明除DNA甲基化以外的次要因素参与Tam3转座的抑制。

项目成果

Nagano Hironori: "Characterization of the repetitive sequences in a 200-kb region around the rice waxy locus, diversity of transposable elements and presence of veiled repetitive sequences"Genes & Genetic Systems. 77(2). 69-79 (2002)

Nagano Hironori：“水稻蜡质基因座周围 200 kb 区域重复序列的特征、转座元件的多样性以及隐藏重复序列的存在”基因

Takagi Kyoko: "MITE-transposon display efficiently detects polymorphisms among the Oryza AA-aenome species"Breeding Science. (In press). (2003)

Takagi Kyoko：“MITE 转座子展示可有效检测水稻 AA 基因组物种之间的多态性”《育种科学》。

K. Takagi, H. Nagano, Y. Kishima, Y. Sano: "MITE-transposon display efficiently detects polymorphisms among the Oryza AA-genome species"Breeding Science. in press.

K. Takagi、H. Nagano、Y. Kishima、Y. Sano：“MITE 转座子展示可有效检测 Oryza AA 基因组物种之间的多态性”《育种科学》。

H. Nagano, M. Kunii, T. Azuma, Y. Kishima, Y. Sano: "Characterization of the repetitive sequences in a 200-kb region around the rice waxy locus : diversity of transposable elements and presence of veiled repetitive sequences"Genes & Genetic Systems. 77. 6

H. Nagano、M. Kunii、T. Azuma、Y. Kishima、Y. Sano：“水稻蜡质基因座周围 200 kb 区域重复序列的特征：转座元件的多样性和隐藏重复序列的存在”基因

Ken Kitamura: "Position effect of the excision frequency of the Antirrhimun transposon Tam3 : implications for the degree of position-dependent methylation in the ends of the element"Plant Molecular Biology. 47. 475-490 (2001)

Ken Kitamura：“金鱼草转座子 Tam3 切除频率的位置效应：对元件末端位置依赖性甲基化程度的影响”植物分子生物学。