Evaluation of the genome stability in the rice cultivars based on indication of the transposable elements

基于转座元件指示的水稻品种基因组稳定性评价

• 批准号: 18580001
• 负责人: KISHIMA Yuji
• 金额: $ 2.51万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580001/
• 关键词: rice; wild rice; transposase gene; tranposon; genetic variations; koshihikari; Oryza; 可動性転移因子; ゲノム; 遺伝変異; 産地間差異; トランスポゾンディスプレイ; mping

Recently, active transposable elements have been abundantly found in plants. Thus, our understanding in evolutionary aspects has accumulated information on the genetic variations caused by transposable elements. However, most of these genetic variations have been described on the genome diversity during speciation. This study was focused on the genetic variations within the cultivar of rice. We have circumstantial evidence of the transpositions of the rice transposon, m-Ping, within the premier rice cultivar, Koshihikari, which was established more than 50 years ago and wildly distributed in the whole Japan. The Koshihikari samples were collected from 84 locations from Fukushima to 14 prefectures in the North. A total of 36 polymorphisms were observed in these Koshihikari collections by transposon display using m-Ping primers. Among these collections, four were obviously originated from other than Koshihikari because the other transposons also showed the similar polymorphisms with m-Ping. It was strongly assumed that the other 32 types are the m-Ping transpositions, which occurred after the Koshihikari was bred. The results represent that transpositions of the transposons can readily inherit to the next generation, and the produced genetic variations are maintained in the different populations within the same cultivars.

近年来，在植物中大量发现活性转座因子。因此，我们对进化方面的理解已经积累了关于转座因子引起的遗传变异的信息。然而，大多数这些遗传变异已经在物种形成过程中的基因组多样性上得到了描述。这项研究的重点是水稻品种内的遗传变异。我们有间接证据证明水稻转座子 m-Ping 在首要水稻品种越光 (Koshihikari) 中发生了转位，该品种于 50 多年前建立，广泛分布于全日本。越光样本是从福岛到北方 14 个县的 84 个地点采集的。通过使用 m-Ping 引物的转座子展示，在这些 Koshihikari 收集品中总共观察到 36 个多态性。在这些集合中，有四个转座子明显来自Koshihikari以外的地方，因为其他转座子也表现出与m-Ping相似的多态性。人们强烈认为其他 32 种类型是 m-Ping 转座，发生在 Koshihikari 培育之后。结果表明，转座子的转座可以容易地遗传到下一代，并且所产生的遗传变异在同一品种的不同群体中得以维持。

项目成果

Acoordinate relationship between transposon Tam3 and the host plant.

转座子 Tam3 与寄主植物之间的协调关系。

• 发表时间: 2007
• 期刊: IDEN, Suppl 21
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako;Uchiyama Takako;Takata Miwako;Noro Yuji;内山 貴子;貴島祐治;Noro Yuji;Uchiyama Takako;Kishima Yuji
• 通讯作者: Kishima Yuji

Rice transposable elements are characterized by various methylation environments in the genome.

• DOI: 10.1186/1471-2164-8-469
• 发表时间: 2007-12-20
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Takata M;Kiyohara A;Takasu A;Kishima Y;Ohtsubo H;Sano Y
• 通讯作者: Sano Y

遺伝 別冊21

遗传学单独卷21

• 发表时间: 2007
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako;Uchiyama Takako;Takata Miwako;Noro Yuji;内山 貴子;貴島祐治;Noro Yuji;Uchiyama Takako;Kishima Yuji;内山 貴子;Noro Yuji;Kishima Yuji;Yuji Kishima;Kishima Yuji;Yuji Kishima;貴島祐治
• 通讯作者: 貴島祐治

Multiple regulatory mechanisms influence the activity of the transposon, Tam3, of Antirrhinum.

多种调控机制影响金鱼草转座子 Tam3 的活性。

• 发表时间: 2008
• 期刊: New phytologist (In press)
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako
• 通讯作者: Uchiyama Takako

Assorted controls of transposon Tam3 in Antirrhinum.

金鱼草中转座子 Tam3 的各种对照。

• 发表时间: 2007
• 期刊: Experimental Medicine 25
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako;Uchiyama Takako;Takata Miwako;Noro Yuji;内山 貴子;貴島祐治;Noro Yuji;Uchiyama Takako
• 通讯作者: Uchiyama Takako