Regulation mechanisms of host cell cycle by baculovirus infection

杆状病毒感染对宿主细胞周期的调控机制

• 批准号: 13660059
• 负责人: IKEDA Motoko
• 金额: $ 2.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660059/
• 关键词: baculovirus; AcMNPV; Sf9 cell; cell cycle; apoptosis; 核多角体病ウイルス

Infection of Autographa californica M nucleopolyhedrovirus (AcMNPV) in Sf9 cells induces cell cycle arrest at S and G2/M phases. To understand the mechanisms of the cell cycle arrest, identification of virus genes responsible for cell cycle arrest was performed. And also changing patterns of several cellular factors responsible for cell cycle regulation were analyzed in Sf9 cells during AcMNPV infection by using several antibodies against those homologues in other animals.

链纹夜蛾核型多角体病毒感染Sf9细胞后，细胞周期发生明显的S期和G2/M期阻滞。为了了解细胞周期停滞的机制，对负责细胞周期停滞的病毒基因进行了鉴定。并利用其他动物中几种抗AcMNPV同源物的抗体，分析了在AcMNPV感染过程中Sf9细胞中负责细胞周期调节的几种细胞因子的变化规律。

项目成果

Iwahori, S.: "Generation and characterization of Autographa californica nucleopolyhedrovirus mutants defective in pcna gene homologue"J. Insect Biotechnol. Sericol.. 71. 129-130 (2002)

Iwahori, S.：“pcna 基因同源物缺陷的苜蓿银纹夜蛾核多角体病毒突变体的生成和表征”J.

Kamiya, K.: "Genotypic variation of a wild isolate of Hyphantria cunea nucleopolyhedrovirus"J. Insect Biotechnol. Sericol.. (in press).

Kamiya, K.：“美国白蛾核多角体病毒野生分离株的基因型变异”J.

Ishikawa,H.: "Induction of apoptosis in IPLB-Ld652Y cell line infected with nucleopolyhedroviruses"J.Gen.Virol.. in press.

Ishikawa, H.：“感染核多角体病毒的 IPLB-Ld652Y 细胞系中诱导细胞凋亡”J.Gen.Virol.. 正在出版。

Felipe Alves, C.A.: "Identification and characterization of Hyphantria cunea nucleopolyhedrovirus homologous repeated regions"Virus Genes. 25. 281-290 (2002)

Felipe Alves，C.A.：“美国白蛾核多角体病毒同源重复区的鉴定和表征”病毒基因。

Kamiya, K.: "Genotypic variation of a wild isolate of Hyphantria cunea nucleopolyhedrovirus"J.Insect Biotechnol.Sericol.. (in press).

Kamiya, K.：“美国白蛾核多角体病毒野生分离株的基因型变异”J.Insect Biotechnol.Sericol..（出版中）。