Degradation of rRNA in insect cells antiviral immune response

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Previously, we demonstrated that rRNA degradation occurs in Bombyx cells (BM-N cells) during abortive infection with heterologous nucleopolyhedroviruses (NPVs). rRNA degradation is likely linked to the global protein synthesis shutdown observed in BM-N cells infected with heterologous NPVs. In the present study, we examined the molecular mechanisms of rRNA degradation of BM-N cells. We cloned cDNAs of RNase and RNase inhibitor from BM-N cells possibly responsible for rRNA degradation, and functional analyses were performed by the transient gene expression and RNAi-based gene silencing. We also examined the possible Bombyx mori NPV factor(s) responsible for inhibition of rRNA degradation in BM-N cells.