权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Environmental acclimation of molluscan are related to a new mechanism for economizing energy

软体动物的环境适应与节约能源的新机制有关

基本信息

批准号：
13660196
负责人：
ENOMOTO Toshiki
金额：
$ 2.3万
依托单位：
Ishikawa Agricultural College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Some glycolytic metabolites in the adductor muscle were measured after transfer of scallops from aerobic to anaerobic saltwater for 12 h. The level of octopine increased gradually during the initial 3h incubation, and thereafter the level increased rapidly up to 12 h. The ATP level also did not show any significant change for the initial 3 h, and then decreased rapidly. In the short-term effects of anaerobiosis for 90 min, the level of Fru 6-P increased just after transfer to anaerobiosis, and then its level decreased. In contrast, Fru 1,6-BP level increased gradually, at the time when both Glc 6-P and Fru 6-P decreased. The AMP was accumulated drastically in contrast with the decrease of ATP. When the same experiments were done by using clams, change in the AMP level reflected those of ATP, and the AMP level in anaerobic clams was about twice as high as in aerobic one at the end of acclimation. These results strongly suggest that molluscan PFK exhibits a low degree of specificity for phosphate donors. Thus, PFK was purified to homogeneity from adductor muscle of scallops, and specificity for phosphate donors was studied. Scallop PFK exhibits a low degree of specificity for phosphate donors, especially ADP. Based on partial amino acid sequences of the purified PFK, it was tried that cDNA of the enzyme is cloned. Complete primary structure of the PFK determined by cDNA cloning remains to be examined.

将扇贝从好氧海水中转移到厌氧海水中12 h后，在闭壳肌中测定了糖酵解代谢产物。章鱼碱的水平在最初的3 h内逐渐增加，此后迅速增加，直至12 h。ATP水平也没有显示出任何显着的变化，在最初的3小时，然后迅速下降。在90 min的短期厌氧作用中，转入厌氧后，Fru 6-P水平升高，随后下降。而Fru 1，6-BP水平逐渐升高，此时Glc 6-P和Fru 6-P均下降。与ATP减少相反，AMP大量积累。当用蛤蜊进行同样的实验时，AMP水平的变化反映了ATP的变化，并且在驯化结束时，无氧蛤蜊中的AMP水平约为有氧蛤蜊中的两倍。这些结果强烈表明，软体动物PFK表现出低程度的磷酸盐供体的特异性。因此，从扇贝闭壳肌中纯化了PFK，并研究了其对磷酸盐供体的特异性。扇贝PFK对磷酸盐供体，尤其是ADP表现出低程度的特异性。根据纯化的PFK的部分氨基酸序列，尝试克隆该酶的cDNA。通过cDNA克隆确定的PFK的完整一级结构仍有待研究。