Elucidation of vasoreactive diversity induced by bradykinin -studies of G-proteins function and bradykinin metabolism -

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(1)Nitric oxide (NO) is spontaneously released from cultured porcine basilar arterial endothelial cells under no stimulation conditions. Bradykinin (BK) enhanced the NO production in a concentration-dependent manner. The enhanced NO production was inhibited by treatments of a selective B_2 receptor antagonist, HOE140, or a NO synthase inhibitor, L-nitro-arginine.(2)Angiotensin (Ang) II induced a contraction in isolated porcine basilar arterial ring. A selective B_2 antagonist, HOE140, inhibited the AngII-induced contraction, while an Ang converting enzyme (ACE) inhibitor, captopril, enhanced the Ang II-induced contraction.(3)BK induces endothelium-dependent relaxation following by contraction in isolated porcine basilar arterial ring. In the presence of L-nitro-arginine (10^<-4> M), BK induced contraction but not relaxation. The BK-induced contraction was not inhibited by a selective AT_1 receptor antagonist, losartan.These results suggest that there might be some interaction between bradykinin and Ang receptors. Further experiments appears to be needed in this point.