A role of amino polysaccharide on extracellular matrix formation in wound healing process

氨基多糖对伤口愈合过程中细胞外基质形成的作用

• 批准号: 13660317
• 负责人: OKAMOTO Yoshiharu
• 金额: $ 1.15万
• 依托单位: TOTTORI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660317/
• 关键词: chitin; chitosan; Wound healing; Extra-cellular matrix; Fibroblasts; Collagen; Type I collagenase

1.Effects of chitin and chitosan on glycosaminoglycan (GAG) and proteoglycan (PG) synthesis : We evaluated effects of chitin and chitosan on GAG and PG synthesis with special staining (Safranin O stain for PG and Alcian blue stain for GAG) and image analyzing using granulation tissue induced by chitin and chitosan. Chitin was found to increase GAG synthesis, while to decrease PG synthesis.2.Effects of molecular weight and deacetylation on wound healing by chitin/chitosan : We studied effects of molecular weight and deacetylation (DAC) on wound healing by chitin/chitosan using linear incisional wound model in rats. We measured break strength of the wound and collagenase activity in the tissue as an indicator of wound healing. Results were as follows :1)Effect of molecular weght : Wound break strengths in all samples were increased significantly compared to that of control (saline). In both chitin (GlcNAc, NACOS, chitin) and chitosan (GlcN, COS, chitosan) groups, oligomers (NACOS, COS) w … More ere most effective. When the chitin and chitosan groups were compared, the chitosan group was higher than the chitin group in each molecular size. Collagenase activities in all samples were increased significantly compared to that of control (saline). In the chitosan group, monomer (GlcN) was most effective, while there was no difference in the chitin group.2)Effect of deacetylation : The levels of both paramers in all samples were significantly higher than that of control. The higher degree of deacetylation showed, the stronger break strength and more collagenase activity. In histologically, more activated fibroblasts were observed in the sample with higher degree of deacetylation.3.Effects of chitin and chitosan on release of type I collagenase (MMP-1) of fibroblasts : We evaluated effects of chitin and chitosan on release of type I collagenase (MMP-l) of fibroblasts using adult human fibroblast (HSF39) and neonatal human fibroblasts (HFFWC, NHDF, HFF11). In all cells, MMP-1 activity in the medium was highest at 72 hrs after incubation. Levels of MMP-1 in the medium were enhanced significantly in the presence of interleukin-1 (IL-1) in HSF39 and HFF11, while those level in HFFWC and NHDF was not affect by IL-1. All samples without G1cN increased significantly release of MMP-l from HSF39, while there was no significant change in MMP-1 level in the presence of all samples in HFFWC. The present study suggests that induction of MMP-l is dependent on origin of fibroblasts. From the fact that oligomers and monomers of chitin and chitosan influenced MMP-1 activity in HSF39, it was suggested that MMP-1 activity continue until finish of complete biodegradation of chitin and chitosan. Less

1。几壳素和壳聚糖对糖胺聚糖（GAG）和蛋白聚糖（PG）合成的影响：我们评估了壳蛋白和壳聚糖对GAG和PG合成的影响，并使用特殊染色（Safranin o STAIN O STAIN O STAIN O STAIN O STAIN pG和ALCIAN BLUETION for GAG）和图像分析的Grans和Chit in Inding and Chits and chit and in Inding and chit inding and chits inding and chit。发现几丁质增加了插入术的合成，而降低了PG合成2。分子量和脱乙酰基化对壳蛋白/壳聚糖的伤口愈合的影响：我们研究了使用大鼠中线性切口伤口模型的分子量和脱乙酰基化（DAC）对壳蛋白/壳聚糖伤口愈合的影响。我们测量了组织中获胜和胶原酶活性的断裂强度，作为伤口愈合的指标。结果如下：1）与对照（盐水）相比，所有样品的伤口断裂强度显着提高。在几丁质（GlcNac，Nacos，Chitin）和壳聚糖（Glcn，Cos，Cos，壳聚糖）组中，低聚物（NACOS，COS）W…更有效。当比较几丁质和壳聚糖基团时，壳聚糖组高于每个分子大小中的几丁质组。与对照（盐水）相比，所有样品中所有样品的胶原酶活性均显着增加。在壳聚糖组中，单体（GLCN）是最有效的，而几丁质组没有差异。2）脱乙酰基化的影响：所有样品中两个参数的水平都显着高于对照。较高程度的脱乙酰基化表现出更强的断裂强度和更多的胶原酶活性。在组织学上，在样品中观察到更高程度的脱乙酰基化的成纤维细胞。3。成纤维细胞对I型胶原酶（MMP-1）释放的壳蛋白和壳聚糖的效应：我们评估了壳聚糖和壳聚糖对I型胶原酶（MMP-lunths and fibib-liflabs of fibib and）释放的影响。新生儿人成纤维细胞（HFFWC，NHDF，HFF11）。在所有细胞中，培养基孵育后72小时的MMP-1活性最高。在HSF39和HFF11中白细胞介素-1（IL-1）的存在下，培养基中的MMP-1水平显着提高，而HFFWC和NHDF中的MMP-1水平不受IL-1的影响。所有没有G1CN的样品显着增加，即从HSF39释放MMP-L，而HFFWC中所有样品的存在，MMP-1水平没有显着变化。本研究表明，MMP-L的诱导取决于成纤维细胞的起源。从几丁质和壳聚糖的低聚物和单体影响HSF39中的MMP-1活性的事实中，建议MMP-1活性一直持续到完全生物降解的几根和壳聚糖为止。较少的

项目成果

Okamoto, Y., et al.: "Mechanism of wound healing acceleration by chitin and chitosan."Res.Adv. in Macromolecules. 3. 1-22 (2002)

Okamoto, Y. 等人：“甲壳素和壳聚糖加速伤口愈合的机制。”Res.Adv。

Okamoto, Y., et al.: "Effects of chitin/chitosan and their oligomers/monomers on migrations of macrophages."Macromol.Biosci.. 3. 587-590 (2003)

Okamoto, Y., et al.：“几丁质/壳聚糖及其低聚物/单体对巨噬细胞迁移的影响。”Macromol.Biosci.. 3. 587-590 (2003)

KOJIMA, K., OKAMOTO, Y., KOJIMA, K., MIYATAKE, K., FUJISE, H., SHIGEMASA, Y., MINAMI, S.: "Effect of chitin and chitosan on prolyl hydroxylase activity."J.Vet.Med.Sci.. (in press).

KOJIMA, K.、OKAMOTO, Y.、KOJIMA, K.、MIYATAKE, K.、FUJISE, H.、SHIGEMASA, Y.、MINAMI, S.：“几丁质和壳聚糖对脯氨酰羟化酶活性的影响。”J.Vet

Okamoto, Y., et al.: "Effects of chitin and chitosan on blood coagulation"Carbohydr. Polym.. (in press).

Okamoto, Y., et al.：“甲壳素和壳聚糖对血液凝固的影响”碳水化合物。

OKAMOTO, Y., YANO, R., MIYATAKE, K., IMAGAWA, T., SHIGEMASA, Y., MINAMI, S.: "Effects of chitin and chitosan on blood coagulation."Carbohydr.Polym.. 53. 337-342 (2003)

OKAMOTO, Y., YANO, R., MIYATAKE, K., IMAGAWA, T., SHIGEMASA, Y., MINAMI, S.：“几丁质和壳聚糖对血液凝固的影响。”碳水化合物。聚合物.. 53. 337-