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Studies on the epidemiology (water birds) for the multiple contaminations by various toxic heavy metals and the effects of the metals on gene.

多种有毒重金属多重污染的流行病学（水禽）研究及其对基因的影响。

基本信息

批准号：
13660328
负责人：
UEDA Fukiko
金额：
$ 2.18万
依托单位：
Nippon Veterinary and Animal Science University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

项目摘要

1.Epidemiological study : Contents of Cr, Mo, Ti, Tl, Cd, V and Cu were investigated for the livers and kidneys of the 74 water birds ; 58 dabbling ducks and 16 diving ducks. The contents for these elements were distributed in wide ranges from ND(not detect value) to more than 10μg/g dry weight except for Cu. Cu contents were very high in many livers of these ducks (more than 100μg/g dry weight), and the contents were also distributed in wide range but had no ND value. Further, several mean values for element contents were largely different from the median values such as Tl contents in the kidneys of widgeons, Ti and V contents in the kidneys and livers of spotbill ducks, respectively. However, we could not jump to a conclusion as the contamination by these metal contents because of no information for the normal range (control) of the metal contents in these ducks. It is generally very difficult to know the exact age, feeding habit and/or living area of each bird species, and therefore … More , it is also difficult to grasp the exact contamination of living area. Thus, we selected Cd pollution having many data, and tried to make a new indicator (an integrated control) for birds and mammals to evaluate of the pollution.2.A new indicator : Cd contents in kidney and liver (83 data) were first obtained from references for wildlife that any special contamination was not written, and then, a Cd standard regression line(CSRL) was made as an integrated control line. The CSRL was verified from the relation between the Cd burdens of normal human and Itai-Itai disease patients, and from the relation between the contents in the organs of normal animals and the experimental animals Cd exposed. The results suggested the availability of CSRL as an integrated control line. Further, this CSRL suggested that 74 water birds investigated in the present study included several contaminated birds.3.Effects of toxic metals on the chromosomal DNA : Effects of Na_3V_4,CdCl_2 and SnCl_2 on chromosomal DNA were investigated using E.coli (K-12 strain) and Lmonocytogenes (L.m ; L98-82B1 strain). The results showed the possibilities that several different mutation sites are located on the chromosomal DNA depending on the metals, and that the mutations occur within short times when the elements exist in the environment. These points are important for the analysis of genomic structures and pathogenicity between the different strains. Thus, further investigation would be needed for these points.4.Conclusion : A part of these results has already published as next page and all results have been summarized in the report submitting with this paper. Less

1.流行病学研究：对74只水鸟、58只水鸭和16只潜水鸭的肝脏和肾脏中Cr、Mo、Ti、Tl、Cd、V和Cu的含量进行了测定。除Cu外，其它元素的含量分布范围较广，从ND（未检出值）到10μg/g干重以上。结果表明，这些鸭肝脏中Cu含量较高（> 100μg/g干重），且分布范围较广，但无ND值。此外，一些元素含量的平均值与中位数有很大的差异，如在肾脏中的宽，Ti和V的含量分别在肾脏和肝脏中的斑嘴鸭，。然而，由于没有这些鸭子中金属含量的正常范围（对照）的信息，我们无法得出这些金属含量污染的结论。一般来说，我们很难知道每种雀鸟的确实年龄、进食习惯及/或生活区，因此， ...更多信息，也很难掌握生活区的确切污染情况。因此，我们选择了数据量较大的Cd污染，尝试建立一个新的鸟类和哺乳动物的Cd污染评价指标（综合控制）。2.新指标：肾脏和肝脏中Cd含量（83个数据），首先从未记载任何特殊污染的野生动物参考文献中获得，然后建立Cd标准回归线（CSRL）作为综合控制线。从正常人和Itai-Itai病患者的Cd负荷之间的关系以及正常动物和实验动物Cd暴露的器官中的含量之间的关系来验证CSRL。结果表明，CSRL作为综合控制线的可用性。3.有毒金属对鸟类染色体DNA的影响：以大肠杆菌（K-12株）和单核细胞增生李斯特菌（L.m.; L98- 82 B1株）为材料，研究了Na_3V_4、CdCl_2和SnCl_2对鸟类染色体DNA的影响。结果表明，根据金属的不同，染色体DNA上可能存在几个不同的突变位点，并且当元素存在于环境中时，突变会在短时间内发生。这对分析不同菌株的基因组结构和致病性具有重要意义。结论：部分结果已发表于下页，所有结果已汇总于随本文提交的报告中。少