Study on the epidemiology of Cadmium contamination in wild birds

1)采用电感耦合等离子体（ICP）法测定了野生鸟类肝脏和肾脏中8种重金属Cd的含量，并对其作为环境污染监测指标的可行性进行了评价。这些鸟类，因此，建议发挥的作用，作为环境污染的监测，因为一些结果获得了某些元素之间的相关性，元素的多重污染，以及污染程度之间的关系和繁殖区，食物或生活场所等，这些结果中的大多数已经发表为11。2）以市售肉类和野生鸟类中单核细胞增生李斯特菌（L.m）的污染情况为基础，研究了重金属和细菌的复合污染以及金属对基因的影响。不同血清型的多重污染，然后，建议在市售肉类，但不是在野生动物。此外，分离的L.m菌株在含重金属（Cd、V、Sn）的培养基中多次培养后，提取染色体DNA。本研究结果为从酶切模式的变化研究重金属对基因的影响奠定了基础。3）采用离体器官和培养细胞研究了重金属的体外吸收。本文阐明了测定困难的V污染豚鼠结肠带中V含量与效应之间的关系。以V为指示剂，研究了金属离子对培养细胞（Vero细胞）的影响，并与离体器官的结果进行了比较。然后，在Vero细胞中获得了类似的结果，尽管反应比离体器官中的反应快。这些结果已部分公布为11。参考文献，更多的研究正在继续进行。

1) The contents of 8 heavy metals containing Cd in the liver and kidney of wild birds were firstly determined with ICP method in the present study, and then it was evaluated whether they were suitable or not as the monitor of environmental contamination. These birds were, consequently, suggested to play a role as an monitor of environmental contamination because several results were obtained for the correlation among certain elements, multiple contamination by elements, and the relations between the degree of the contamination and reproduction area, foods or living sites etc. Most of these results have already been published as 11. REFERENCES (partly, in submitted for publication).2) The contamination by L.monocytogense (L.m) in marketed meets and wildlife containing bird were searched as the base study for the investigations of multiple contamination by heavy metals and bacteria, and effects of metals on gene. Multiple contamination by different serotypes was, then, suggested in the marketed meats but not in wildlife. Further, chromosomal DNAs were extracted after the several times' cultures of isolated L.m strains in the medium containing heavy metals (Cd, V, Sn). In the results the base was established for the investigation of the effects of heavy metals on gene from the change in endonuclease cleveage patterns.3) The uptake of heavy metals was investigated in vitro using isolated organs and culture cell. The relation between the V contents and effects were clarified in guinea-pig taenia coli for the contaminated V which measurement was known to be difficult. Further, the effects of metals on cultured cell (Vero cell) were investigated using V as indicator, then the results were compared with them from isolated organs. Similar results were, then, obtained in the Vero cell though the reaction was faster than those in isolated organs. These results have been partially published as 11. REFERENCES, and more study is in continued examination.