Electrophysiological analysis of feeding-related network in the hypothalamus, based on the gluco-static and lipo-static theories

基于葡萄糖静态和脂肪静态理论的下丘脑摄食相关网络的电生理分析

• 批准号: 13670056
• 负责人: SASAKI Kazuo
• 金额: $ 2.56万
• 依托单位: Toyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670056/
• 关键词: Leptin; Ghrelin; Orexin; Arcuate nucleus; Rat; カルシウム; 室傍核

1. In brain slice experiments, neuronal activity of the ventro-medial part of the arcuate nucleus (mARC) in rats was excited dose-dependently by application of leptin. The ratio of neurons excited by 10^<-10>M leptin was about 60%. The excitation was observed in low Ca^<2+>, high Mg^<2+> Ringer's solution as well as in normal Ringer's solution.2. Neuronal activity of the mARC was excited dose-dependently by application of orexin-A. The ratio of mARC neurons excited by 10^<-7>M orexin-A was about 70%. The excitation was observed in low Ca^<2+>, high Mg^<2+> Ringer's solution as well as in normal Ringer's solution. Neurons excited by orexin-A were also excited by orexin-B. The magnitude of excitation induced by orexin-B was greater than that induced by orexin-A.3. Neuronal activity of the mARC was excited dose-dependently by ghrelin. The ratio of mARC neurons excited by 10^<-7>M ghrelin was about 70%. The excitation was observed in low Ca^<2+>, high Mg^<2+> Ringer's solution as well as in normal Ringer's solution. About a half of neurons excited by ghrelin was also excited by orexin.

1.在脑切片实验中，大鼠弓状核腹内侧部（mARC）的神经元活动被剂量依赖性地兴奋。10 μ M瘦素可兴奋神经元的比例<-10>约为60%。在低Ca^&lt;2+&gt;、高Mg^&lt;2+&gt;的林格氏液和正常的林格氏液中均观察到这种兴奋.通过应用食欲素A，mARC的神经元活性被剂量依赖性地激发。10 μ M orexin-A兴奋的mARC神经元的比例<-7>约为70%。在低Ca^&lt;2+&gt;、高Mg^&lt;2+&gt;的林格氏液和正常的林格氏液中均观察到这种激发。食欲素-A兴奋的神经元也被食欲素-B兴奋。orexin-B诱导的兴奋幅度大于orexin-A诱导的兴奋幅度。mARC的神经元活性被ghrelin剂量依赖性地激发。10 μ M ghrelin兴奋mARC神经元的比例<-7>约为70%。在低Ca^&lt;2+&gt;、高Mg^&lt;2+&gt;的林格氏液和正常的林格氏液中均观察到这种激发。大约有一半的神经元被ghrelin兴奋，也被orexin兴奋。

项目成果

Demonstration of acidic fibroblast growth factor (FGF-1) in rat adrenal gland.

大鼠肾上腺中酸性成纤维细胞生长因子 (FGF-1) 的演示。

• 发表时间: 2001
• 期刊: Acta Histochem. Cytochem. 34
• 作者: Matsuoka Yasuji;et al.
• 通讯作者: et al.

Yasui Fumihiko: "Antioxidant and/or prooxidant activities of carnitine and its derivative on the hydroxyl radical generation by the Fenton reaction"ITE Lett.on Batt.New Technol. & Med.. 3. 58-62 (2002)

安井文彦：“肉碱及其衍生物对芬顿反应产生羟基自由基的抗氧化和/或促氧化活性”ITE Lett.on Batt.New Technol。

Matsuoka Yasuji: "Demonstration of acidic fibroblast growth factor (FGF-1) in rat adrenal gland"Acta Histochem. Cytochem.. 34・2. 129-134 (2001)

松冈安司：“大鼠肾上腺中酸性成纤维细胞生长因子（FGF-1）的演示”细胞化学学报.. 129-134（2001）

Antioxidant and/or prooxidant activities of carnitine and its derivative on the hydroxyl radical generation by the Fenton reaction.

肉碱及其衍生物对芬顿反应产生羟基自由基的抗氧化和/或促氧化活性。

• 发表时间: 2002
• 期刊: ITE Lett. on Batt. New Technol. & Med. 3
• 作者: Yasui Fumihiko;Yasui Fumihiko
• 通讯作者: Yasui Fumihiko

Yasui Fumihiko: "Effect of chronic acetyl-L-carnitine treatment on brain lipid hydroperoxide level and passive avoidance learning in senescence-accelerated mice"Neurosci.Lett.. 334. 177-180 (2002)

安井文彦：“慢性乙酰左旋肉碱治疗对衰老加速小鼠脑脂质氢过氧化物水平和被动回避学习的影响”Neurosci.Lett.. 334. 177-180 (2002)