Effects of acidic fibroblast growth factor released by feeding on learning and memory, endocrine and immune systems

1.碱性成纤维细胞生长因子及其片段对学习记忆的影响：众所周知，衰老加速小鼠(SAMP8)学习记忆能力下降。在本研究中，SAMP8接受S.C.注射成纤维细胞生长因子或其片段。这些注射是在21日龄断奶后开始的，每周重复一次，持续10个月。在被动回避实验和Morris水迷宫实验中，aFGF或其片段可显著改善SAMP8大鼠的学习记忆能力。结论：1.aFGF及其片段对SAMP8大鼠学习记忆功能的改善作用可能与其保护内侧隔胆碱能神经元的功能有关。碱性成纤维细胞生长因子对内分泌系统的影响大鼠注射aFGF后，血浆皮质酮和肾上腺素水平明显升高。静脉注射前病毒。在注射aFGF前注射抗CRF抗体可消除血浆皮质酮和肾上腺素水平的升高。因此，可以认为aFGF通过CRF.3激活下丘脑-垂体-肾上腺皮质轴和肾上腺髓质的分泌。碱性成纤维细胞生长因子及其片段对免疫系统的影响：用流式细胞仪检测其对大鼠腹腔巨噬细胞吞噬功能的影响。结果表明，aFGF1-20和aFGF1-29呈剂量依赖性地激活巨噬细胞的吞噬功能。

1. Effect of aFGF and its fragment on learning and memory : It is well known that senescence-accelerated mice (SAMP8) have a deteriorated ability on learning and memory. In the present study, SAMP8 received s.c. injections of aFGF or its fragment. The injections were started after weaning at 21 days of age and repeated once a week for 10 months. Consequently, aFGF or its fragment significantly improved the ability on learning and memory of SAMP8, when examined with passive avoidance test and Morris water maze test. The beneficial effect of aFGF and its fragment on learning and memory function in SAMP8 may be related to a preservation of function in medial septum cholinergic neurons.2. Effect of aFGF on endocrine system : I.c.v. injections of aFGF in rats significantly increased plasma corticosterone level and plasma adrenaline level. Pre-I.c.v. injections of anti-CRF antibody before aFGF injections abolished the increase of plasma corticosterone and adrenaline levels. Thus, it may be possible to state that aFGF activates hypotlhlamic-pituitary-adrenocortical axis and adrenomedullary secretion through CRF.3. Effect of aFGF and its fragments on immune system : Action of aFGF and its fragments on phagocytosis of peritoneal macrophages in rats was investigated by flow cytometry. The results indicate that aFGF, aFGF(1-20) and aFGF(1-29) dose-dependently activate phagocytosis of macrophages.