The role of the Cbl/Syk complex in FceRl-induced signaling cascade in mast cells

本研究探讨了Cbl在Fcε RI诱导的肥大细胞信号级联反应中的作用，特别是Cbl中脯氨酸富集区的作用。通过定点突变法对脯氨酸（543-548）进行突变，突变后的Cbl在肥大细胞中表达。Syk的酪氨酸磷酸化水平及Syk与FCεRI γ链的结合水平无明显变化。转染突变型Cbl的细胞NF-κB的活化和组胺的释放较野生型Cbl的细胞略有增加。这些结果共同表明Cbl富含脯氨酸的区域调节肥大细胞中FCε RI诱导的信号级联反应。

The role of Cbl in FcεRI-induced signaling cascade in mast cells were examined, especially focused on the role of proline rich region in Cbl. Proline (543-548) were mutated by a cite direct mutagenesis, and the mutated Cbl was expressed in mast cells. The levels of tyrosine phosphorylation of Syk and association between Syk and γ chain of FCεRI were unchanged. However, activation of transcription factor NF-κB and histamine release were slightly enhanced in cells transfected with the mutated Cbl compared with that in cells transfected with wild type Cbl. These results collectively indicated that proline rich region of Cbl regulates the FCεRI-induced signaling cascade in mast cells.