The role of the Cbl/Syk complex in FceRl-induced signaling cascade in mast cells

Cbl/Syk复合物在FceRl诱导的肥大细胞信号级联中的作用

• 批准号: 13670449
• 负责人: OTA Yasuo
• 金额: $ 2.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670449/
• 关键词: cbl; syk; mast cells; FcεRl; Ubiquitin; Cbl; Syk

The role of Cbl in FcεRI-induced signaling cascade in mast cells were examined, especially focused on the role of proline rich region in Cbl. Proline (543-548) were mutated by a cite direct mutagenesis, and the mutated Cbl was expressed in mast cells. The levels of tyrosine phosphorylation of Syk and association between Syk and γ chain of FCεRI were unchanged. However, activation of transcription factor NF-κB and histamine release were slightly enhanced in cells transfected with the mutated Cbl compared with that in cells transfected with wild type Cbl. These results collectively indicated that proline rich region of Cbl regulates the FCεRI-induced signaling cascade in mast cells.

本研究探讨了Cbl在Fcε RI诱导的肥大细胞信号级联反应中的作用，特别是Cbl中脯氨酸富集区的作用。通过定点突变法对脯氨酸（543-548）进行突变，突变后的Cbl在肥大细胞中表达。Syk的酪氨酸磷酸化水平以及Syk与FCεRI γ链之间的结合没有变化。转染突变型Cbl的细胞NF-κB的活化和组胺的释放较野生型Cbl的细胞略有增加。这些结果共同表明Cbl富含脯氨酸的区域调节肥大细胞中FCε RI诱导的信号级联反应。

项目成果

太田康男: "サイトカイン受容体からの情報伝達"内科. 90(12). 1140-1143 (2002)

Yasuo Ota：“细胞因子受体的信息传输”内科医学90（12）1140-1143（2002）。

Shu Okugawa, Yasuo Ota, et al.: "Janus Kinase 2 is involved in Lipopolysaccharide-Induced Activation of Macrophages"Am J Physiol Cell Physiol. 285. C399-C408 (2003)

Shu Okukawa、Yasuo Ota 等人：“Janus 激酶 2 参与脂多糖诱导的巨噬细胞激活”Am J Physiol Cell Physiol。

太田康男: "抗HIV治療ガイドライン(厚生労働省研究班)"日本臨床新世紀の感染症学 上. 2月. 670-676 (2003)

Yasuo Ota：“抗HIV治疗指南（厚生劳动省研究小组）”日本临床新世纪传染病卷1.2月670-676（2003年）

Shu Okugawa, Yasuo Ota, Takatoshi Kitazawa, Kuniko Nakayama, Shintaro Yanagimoto, Kunihisa Tsukada, Miki Kawada, Satoshi Kimura: "Janus Kinase 2 is involved in Lipopolysaccharide-Induced Activation of Macrophages."Am J Physiol Cell Physiol. 285. C399-C408

Shu Okukawa、Yasuo Ota、Takatoshi Kitazawa、Kuniko Nakayama、Shintaro Yanagimoto、Kunihisa Tsukada、Miki Kawada、Satoshi Kimura：“Janus 激酶 2 参与脂多糖诱导的巨噬细胞激活。”Am J Physiol Cell Physiol。