C.difficileフラジェリンが感染成立に果たす役割の研究

艰难梭菌鞭毛蛋白在感染建立中的作用研究

• 批准号: 20591198
• 负责人: OTA Yasuo
• 金额: $ 3万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591198/
• 关键词: Clostridium difficile; フラジェリン; TLR5; トキシンB; 下痢症; 腸管上皮細胞; NF-κB; C.difficile

C. difficile is an important pathogen of nosocomial infection, and outbreak of C.difficile associated diseases (CDAD) often occurs in hospitals. However, the pathogenesis of CDAD remains to be determined. We investigated whether C. difficile flagellin were involved in the pathogenesis of CDAD. C. difficile flagellin induced activation of NF-kappaB and p38 mitogen-activated Protein Kinase (MAPK) via TLR5, and also promoted productions of Interleukin-8 (IL-8) and CCL20 in intestinal epithelial cells via TLR5. Pretreatment with toxin B stimulation enhanced production of CCL20 induced by C. difficile flagellin by increasing expressions of TLR5 in intestinal epithelial cells. These results collectively indicated that C. difficile flagellin plays an important role in the pathogenesis of CDAD.

C.艰难梭菌是医院感染的重要病原菌，艰难梭菌相关疾病（CDAD）的暴发流行在医院中时有发生。然而，CDAD的发病机制仍有待确定。我们调查了C.艰难梭菌鞭毛蛋白参与CDAD的发病。C.艰难梭菌鞭毛蛋白通过TLR 5诱导NF-κ B和p38丝裂原活化蛋白激酶（MAPK）的活化，还通过TLR 5促进肠上皮细胞中白细胞介素-8（IL-8）和CCL 20的产生。毒素B刺激预处理可增强C.艰难梭菌鞭毛蛋白通过增加肠上皮细胞中TLR 5的表达。这些结果表明，C.艰难梭菌鞭毛蛋白在CDAD的发病机制中起重要作用。

项目成果

Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated isothermal amplification (LAMP) and melting curve analysis

结合 DNA 滤纸、环介导等温扩增 (LAMP) 和熔解曲线分析的新型恶性疟原虫快速分子诊断系统的评价

• 发表时间: 2009
• 期刊: Japanese Journal of Infectious Diseases 62(1)
• 作者: Yamamura M;Makimura K;Ota Y
• 通讯作者: Ota Y

Combined treatment with oral kanamycin and parenteral antibiotics for a case of persistent bacteremia and intestinal carriage with Campylobacter coli

• DOI: 10.2169/internalmedicine.47.1161
• 发表时间: 2008-01-01
• 期刊: INTERNAL MEDICINE
• 影响因子: 1.2
• 作者: Okada, Haruka;Kitazawa, Takatoshi;Koike, Kazuhiko
• 通讯作者: Koike, Kazuhiko

Polymerase chain reaction assay for specific identification of Candida guilliermondii (Pichia guilliermondii).

用于特异性鉴定吉里蒙假丝酵母（吉里蒙毕赤酵母）的聚合酶链反应测定。

• 发表时间: 2009
• 期刊: Infect Chemother. 15(4)
• 作者: Mirhendi H;Moazeni M;Nikaeen M;Makimura K.;Onozaki M;Koga H;Yamada T;Yamamura M;AlShahni MM;Uchida T;Ogawa H;Ogawa H;AlShahni MM;Ebihara M;Sugita T;Ogawa H;Yamamura M
• 通讯作者: Yamamura M

An essential single amino acidof Toll-like receptor 4 that is pivotal for its signaltransduction and subcellular localization

Toll 样受体 4 的必需单一氨基酸，对其信号转导和亚细胞定位至关重要

• 发表时间: 2009
• 期刊: J.Biol Chem 284巻
• 作者: Yanagimoto S;Tatsuno K;Okugawa S;Kitazawa T;Tsukada K;Koike K;KodamaT;Kimura S;Shibasaki Y;Ota Y
• 通讯作者: Ota Y

An essential single amino acidof Toll-like receptor 4 that is pivotal for its signal transduction and subcellular localization.

Toll 样受体 4 的必需单一氨基酸，对其信号转导和亚细胞定位至关重要。

• 发表时间: 2009
• 期刊: J.Biol Chem 254
• 作者: Yanagimoto S;et.al.
• 通讯作者: et.al.