A study on autoantibodies to killer immunoglobulin-like receptors and hyper-gamma-globulinemia in systemic autoimmune diseases

系统性自身免疫性疾病中杀伤性免疫球蛋白样受体自身抗体和高丙种球蛋白血症的研究

• 批准号: 13670483
• 负责人: KATO Tomohiro
• 金额: $ 2.3万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670483/
• 关键词: anti-lymphocyte antibody; NK cell; killer immunoglobulin-like receptor; antibody; systemic lupus erythematosus; Killer cell Immunoglobulin-like Receptor; 慢性関節リウマチ; 強皮症; 全身性エリテマトーデス; シェーグレン症候群

We have been investigating so-called anti-lymphocyte antibodies, antibodies to cell surface molecules on the lymphocytes. In the present study, we tried to detect and analyze autoantibodies to killer immunoglobulin-like receptor 2 DL, one of the NK receptors. We here evidenced existence of autoantibodies to KIR2DL in rheumatoid arthritis, systemic lupus erythematosus, and Behcet's disease, where the existence was correlated with hyper-gamma-globulinemia. To further analyze mechanisms for this, we investigated the effects of the KIR2DL autoantibodies on the anti-cytotoxic activity of NK cells, however, the detected effects were different between the serum samples. Further more detailed analysis would be needed to elucidate the mechanisms.

我们一直在研究所谓的抗淋巴细胞抗体，即针对淋巴细胞表面分子的抗体。在本研究中，我们试图检测和分析NK受体中的一种--杀手免疫球蛋白样受体2DL的自身抗体。我们在此证实，在类风湿性关节炎、系统性红斑狼疮和白塞病中存在KIR2DL自身抗体，这些自身抗体的存在与高丙种球蛋白血症有关。为了进一步分析其机制，我们研究了KIR2DL自身抗体对NK细胞抗细胞毒活性的影响，但检测到的效果在不同的血清样本中是不同的。还需要进行更详细的分析，以阐明这些机制。

项目成果

Okamoto K, Yotsuyanagi H, Ooka S, Matsui T, Suzuki Kurokawa M, Suzuki M, Iino S, Nishioka K, Kato T: "Autoantibodies to CD69 in Patients with Chronic Hepatitis Type C: A Candidate Maker for Predicting the Response to Interferon Therapy"Intervirology. 46.

Okamoto K、Yotsuyanagi H、Ooka S、Matsui T、Suzuki Kurokawa M、Suzuki M、Iino S、Nishioka K、Kato T：“慢性丙型肝炎患者的 CD69 自身抗体：预测干扰素治疗反应的候选指标

Tsumuki H et al.: "Infection of synoviocytes with HTLV-I induces telomerase activity"Rheumatol Int.. Jul;20(5). 175-179 (2001)

Tsumuki H 等人：“用 HTLV-I 感染滑膜细胞会诱导端粒酶活性”Rheumatol Int.. Jul；20(5)。

Pistillo MP, Tazzari PL, Gaudiano C, Cilla V, Kato T, Matsui T, Nishioka K, Capanni P, Conte R, Ferrara GB: "Patients with neoplastic and nonneoplastic hematologic diseasesacquire CTLA-4 antibodies after blood transfusion"Transfusion. 41. 462-469 (2001)

Pistillo MP、Tazzari PL、Gaudiano C、Cilla V、Kato T、Matsui T、Nishioka K、Capanni P、Conte R、Ferrara GB：“肿瘤性和非肿瘤性血液疾病患者在输血后获得 CTLA-4 抗体”输血。

Kurokawa M et al.: "Paired-cloning of the T cell receptor α and β genes from a single T cell without the establishment of a T cell clone"Clin. Exp. Immunol.. 123. 340-345 (2001)

Kurokawa M 等人：“在不建立 T 细胞克隆的情况下从单个 T 细胞配对克隆 T 细胞受体 α 和 β 基因” Clin Exp. 123. 340-345 (2001)

Kurokawa M, Kato T, Nishioka K: "Detection of clonally expanded T cells by RT-PCR-SSCP and nucleotide Sequencing of T cell receptor β-CDR3 regions"Methods Mol. Biol. 193. 267-280 (2001)

Kurokawa M、Kato T、Nishioka K：“通过 RT-PCR-SSCP 和 T 细胞受体 β-CDR3 区域的核苷酸测序检测克隆扩增的 T 细胞”方法分子生物学 193。267-280 (2001)