The Role of the Tumor Necrosis Factor Receptor in 2,4,6-Trinitrobenzene Sulphonic Acid (TNBS)-induced Colitis in Mice

肿瘤坏死因子受体在 2,4,6-三硝基苯磺酸 (TNBS) 诱导的小鼠结肠炎中的作用

基本信息

  • 批准号:
    15590635
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2005
  • 项目状态:
    已结题

项目摘要

It is well known that tumor necrosis factor (TNF) plays a key role in the immunopathogenesis of inflammatory bowel disease (IBD) ; the mechanism, however, has not yet been defined. To elucidate the role of the TNF receptor in IBD, we investigated the effect of administering an enema containing 2,4,6-trinitrobenzene sulphonic acid (TNBS), which produces many histological and immunological conditions similar to those of Crohn's disease, using wild (WT), TNFR-1KO, TNFR-2KO and TNFR-1,2KO C57BL/6 strain mice. Mice were irrigated with 6 mg of TNBS into the colon via the anus and sacrificed one week later. In the histological assessment, inflammatory cell scores showed no significant differences among all TNBS-administered groups, whereas tissue damage scores were significantly lower in TNFR-1KO and TNFR-1,2KO mice than in WT mice. The apoptotic indexes of mononuclear cells in the lamina propria of all TNBS-administered groups assessed by TUNEL straining were significantly lower than that of controls. Serum TNF levels of all TNBS-administered groups did not differ significantly from that of, controls, whereas TNF-a mRNA expression in the colon was significantly higher in all TNBS-administered groups than in controls. Further, NF-kb activities were enhanced in WT and TNFR-2KO mice compared with those in control mice. In conclusion, the present data suggest that continuous infiltration of inflammatory cells is substantially responsible for the pathogenesis of TNBS-colitis in mice, which is closely associated with defective apoptosis of mononuclear cells in the lamina propria but not with the TNF/TNFR signaling system.
众所周知,肿瘤坏死因子(TNF)在炎症性肠病(IBD)的免疫发病机制中起着关键作用,但其机制尚未明确。为了阐明肿瘤坏死因子受体在IBD中的作用,我们用野生型(WT)、TNFR-1KO、TNFR-2KO和TNFR-1,2KO C57BL/6品系小鼠,研究了含有2,4,6-三硝基苯磺酸(TNBS)的灌肠对IBD的影响。小鼠经肛门向结肠内注入6 mg TNBS,一周后处死。在组织学评估中,所有TNBS组的炎性细胞评分没有显著差异,而组织损伤评分在TNFR-1KO和TNFR-1,2KO组显著低于WT组。TUNEL法检测TnBS各剂量组固有层单个核细胞的凋亡指数均显著低于对照组。所有给药组的血清肿瘤坏死因子水平与对照组没有显著差异,而结肠组织中的肿瘤坏死因子-αmRNA表达在所有给药组显著高于对照组。此外,WT组和TNFR-2KO组小鼠的核因子-kb活性均高于对照组。综上所述,目前的数据表明,炎症细胞的持续渗透是小鼠TNBS-结肠炎的重要原因,而与固有层单个核细胞的缺陷凋亡密切相关,而与肿瘤坏死因子/肿瘤坏死因子受体信号系统无关。

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The Role of the Tumor Necrosis Factor Receptor in 2,4,6-Trinitrobenzene Sulphonic Acid (TNBS)-Induced Colitis in Mice.
肿瘤坏死因子受体在 2,4,6-三硝基苯磺酸 (TNBS) 诱导的小鼠结肠炎中的作用。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Minoru Nakai
  • 通讯作者:
    Minoru Nakai
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KATO Tomohiro其他文献

Relationship between social foster care institutional staff's knowledge of behavioral theories and their efficacy in fostering children in residential care.
社会寄养机构工作人员的行为理论知识与其寄养儿童的功效之间的关系。
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    KATO Tomohiro;IMOTO Yukari;YASUTAKA Tetsuo;KATSUMI Takeshi;A. Takii and D. Ito
  • 通讯作者:
    A. Takii and D. Ito
DEVELOPMENT OF NEW MPS-BASED WAVE GENERATION MODEL
基于 MPS 的新型波浪发生模型的开发
Current Status and Prospects of Ground Improvement Technologies for Disaster Prevention and Environmental Conservation
防灾环保地基改良技术现状与展望
Evaluating the performance of attenuation layer using the partition coefficients determined from column sorption test
使用柱吸附测试确定的分配系数评估衰减层的性能
  • DOI:
    10.3208/jgs.16.131
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    KATO Tomohiro;IMOTO Yukari;YASUTAKA Tetsuo;KATSUMI Takeshi
  • 通讯作者:
    KATSUMI Takeshi
Monotonous decreasing leaching behavior of geogenic contamination from marine sediments by up-flow column percolation tests
上流柱渗滤试验对海洋沉积物地源污染的单调递减淋滤行为
  • DOI:
    10.3208/jgs.16.209
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    KATO Tomohiro;IMOTO Yukari;YASUTAKA Tetsuo;KATSUMI Takeshi
  • 通讯作者:
    KATSUMI Takeshi

KATO Tomohiro的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KATO Tomohiro', 18)}}的其他基金

Studies on the role of annexin7 in the pathophysiology and therapies of rheumatoid arthritis using proteomics
利用蛋白质组学研究膜联蛋白7在类风湿关节炎病理生理学和治疗中的作用
  • 批准号:
    20390283
  • 财政年份:
    2008
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Studies on target molecules for anti-endothelial cells autoantibodies detected in systemic vasculitis.
系统性血管炎中抗内皮细胞自身抗体靶分子的研究。
  • 批准号:
    15591069
  • 财政年份:
    2003
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A study on autoantibodies to killer immunoglobulin-like receptors and hyper-gamma-globulinemia in systemic autoimmune diseases
系统性自身免疫性疾病中杀伤性免疫球蛋白样受体自身抗体和高丙种球蛋白血症的研究
  • 批准号:
    13670483
  • 财政年份:
    2001
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A method to determine antigen peptides for the conally expanded T cells in systemic autoimmune diseases
系统性自身免疫性疾病中圆锥扩增 T 细胞抗原肽的测定方法
  • 批准号:
    11670465
  • 财政年份:
    1999
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of antigens for proliferating T cells in patients with systemic lupus erythematosus.
系统性红斑狼疮患者增殖 T 细胞抗原的分析。
  • 批准号:
    09670496
  • 财政年份:
    1997
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了