Analysis of antigens for proliferating T cells in patients with systemic lupus erythematosus.

系统性红斑狼疮患者增殖 T 细胞抗原的分析。

• 批准号: 09670496
• 负责人: KATO Tomohiro
• 金额: $ 1.86万
• 依托单位: St.Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670496/
• 关键词: systemic lupus erythematosus; Tcell; antigen-specific reaction; auto antigen

Many lines of studies suggested that T cells play pivotal roles in the pathogenesis of systemic lupus erythematosus (SLE). We reported oligoclonal T cell proliferation in peripheral blood of patients with the disease previously. To identify the antigen peptides for the accumulated T cell clonotypes, we developed a T cell receptor cloning method from a single cell for the reconstruction of TCR molecules. Further, we successfully expressed HLA class I molecules with beta-2-microglobulin on the filamentous phages as a single chain for constructing an antigen peptide library. Screening of the library with the reconstructed TCR would determine candidate antigens for the proliferating T cells in SLE.

许多研究表明，T细胞在系统性红斑狼疮（SLE）的发病机制中起着关键作用。我们以前曾报道过这种疾病患者外周血中寡克隆T细胞增殖。为了鉴定积累的T细胞克隆型的抗原肽，我们开发了一种从单个细胞克隆T细胞受体的方法，用于TCR分子的重建。此外，我们成功地表达HLA I类分子与β-2-微球蛋白的丝状真菌作为一个单链构建抗原肽库。用重建的TCR筛选文库将确定SLE中增殖T细胞的候选抗原。

项目成果

A.Tanaka, et al.: "Clonotypic Analysis of T cells in Patients With Autoimmune and Viral Hepatitis" Hepatology. 25. 1070-1076 (1997)

A.Tanaka 等人：“自身免疫性和病毒性肝炎患者 T 细胞的克隆型分析”肝病学。

K.Fujisawa, et al.: "Evidence for autoantigens of env/tax protein in HTLV-Ienv -pX transgenic mice." Arthritis & Rheum. 41. 101-109 (1998)

K.Fujisawa 等人：“HTLV-Ienv -pX 转基因小鼠中 env/tax 蛋白自身抗原的证据。”

J.Tong, et al.: "A Novel Subfamily of Hepatitis G Virus/Hepatitis GB virus C Isolated form a japanese patient : A Sequence Analysis of the Envelope(El)Region" Journal of Clinical Microbiology. 36. 2797-2799 (1998)

J.Tong 等人：“从日本患者中分离出的 G 型肝炎病毒/GB 型肝炎病毒的新亚科：包膜 (E1) 区域的序列分析”《临床微生物学杂志》。

T.Sumida I.Matsumoto T.Kato T.Hasunuma T.Maeda K.Nishioka: "Regulatory T Cell Epitope Recognized by T Cells from Labial Salivary Glands of Patients" Arthritis & Rheum. 40. 2271-2273 (1997)

T.Sumida I.Matsumoto T.Kato T.Hasunuma T.Maeda K.Nishioka：“患者唇唾液腺 T 细胞识别的调节性 T 细胞表位”关节炎

K.Fujisawa K.Okamoto H.Asahara T.Hasunuma T.Kobata T.Kato T.Sumida K.Nishioka: "Evidence for autoantigens of env/tax protein in HTLV-I env-pX transgenic mice." Arthritis & Rheum. 41. 101-109 (1998)

K.Fujisawa K.Okamoto H.Asahara T.Hasunuma T.Kobata T.Kato T.Sumida K.Nishioka：“HTLV-I env-pX 转基因小鼠中 env/tax 蛋白自身抗原的证据。”