Specification of the factor in a liver cell required for multiplication of hepatitis C virus

丙型肝炎病毒增殖所需的肝细胞因子的规格

• 批准号: 13670521
• 负责人: CHAYAMA Kazuaki
• 金额: $ 1.92万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670521/
• 关键词: hepatitis C virus; chronic hepatitis; acute hepatitis; full length clone; replicon; HCV infectiousclone; IFN-β; RNA interference; IRFファミリー; polymerase; helicase; protease; polymerase chain reaction

Hepatitis C virus infection is a serious health problem worldwide. As the hepatitis C virus infects only humans and chimpanzees, establishment of reliable culture system is necessary to study this important pathogen. We cloned a full length genotype 1b cDNA clone of the hepatitis C virus from a patient who developed acute hepatitis C. This cDNA clone was used to construct several replicon clones and infectious clones. These constructs should be useful to study the hepatitis C virus.

丙型肝炎病毒感染是一个严重的全球性健康问题。由于丙型肝炎病毒只感染人类和黑猩猩，建立可靠的培养系统是研究这一重要病原体的必要条件。我们克隆了一个全长基因型1b型丙型肝炎病毒的cDNA克隆从病人谁发展急性丙型肝炎。该cDNA克隆用于构建几个复制子克隆和感染性克隆。这些结构应该是有用的研究丙型肝炎病毒。

项目成果

S.Tazuma, T.Nishioka, Chayama K et al.: "Impaired gallbladder mucosal function in aged gallstone patients suppresses gallstone recurrence after successful extracorporeal shockwave lithotripsy"J Gastroenterol Hepatol. 18(2). 157-161 (2003)

S.Tazuma、T.Nishioka、Chayama K 等人：“老年胆结石患者胆囊粘膜功能受损可抑制体外冲击波碎石术成功后胆结石复发”J Gastroenterol Hepatol。

K.Tsuboi, S.Tazuma, H.Ochi, Chayama K.: "Hydrophilic bile salts have a cyclosporine A-induce cholestasis through enhanced canalicular membrane fluidity and transporter activity"Hepatol Res. 25(1). 38-47 (2003)

K.Tsuboi、S.Tazuma、H.Ochi、Chayama K.：“亲水性胆汁盐具有环孢菌素 A-通过增强小管膜流动性和转运蛋白活性诱导胆汁淤积”Hepatol Res。

W.Ohishi, M.Kitamoto, Chayama K.ほか7名: "Impact of aging on the development of hepatocellular carcinoma in patients with hepatitis C virus infection in japan."Scand J Gastroenterol. 38,8. 894-900 (2003)

W.Ohishi、M.Kitamoto、Chayama K. 和其他 7 人：“日本丙型肝炎病毒感染患者中衰老对肝细胞癌发展的影响”，Scand J Gastroenterol 38,8 (2003)。

K.Nakai, S.Tazuma, T.Nishioka, Chayama K.: "Inhibiton of cholesterol crystallization under bilirubin deconjugation: partial characterization of mechanisms whereby infected bile accelerates pigment stone formation."Biochim Biophys Acta. 1632,1-3. 48-54 (20

K.Nakai、S.Tazuma、T.Nishioka、Chayama K.：“胆红素解离作用下胆固醇结晶的抑制：感染胆汁加速色素结石形成机制的部分表征。”Biochim Biophys Acta。

Waka Ohishi, K.Chayama: "Rare Quasispecies in the YMDD motif of Hepatitis B Virus Detected by Polymerase Chain Reaction with Peptitde Nucleic Acid Clamping"Intervirology. 46・6. 355-361 (2003)

Waka Ohishi，K.Chayama：“通过肽核酸钳位的聚合酶链反应检测乙型肝炎病毒 YMDD 基序中的稀有准种”Intervirology 46・6 (2003)。