Analysis of lamivudine resistance acquisition by hepatitis B virus.

B型肝炎病毒感染是世界范围内严重的健康问题。由于B型肝炎病毒只感染人类和黑猩猩，因此建立可靠的培养体系是研究这一重要病原体的必要条件。我们从一个非常轻微的肝炎患者中克隆了一个长度为fu 11的B型肝炎病毒C基因型克隆。将约一个半基因组克隆到质粒载体中。将1.4长度克隆转染至HepG 2细胞系。所得细胞系产生HBs抗原、HBe抗原和HBV DNA至上清液。HBV DNA滴度为10^6-10^7拷贝/ml。拉米夫定和干扰素的孵育减少了HBV DNA的产生。这些克隆将有助于进一步开发抗B型肝炎病毒的药物和研究B型肝炎病毒的生物学特性。

Hepatitis B virus infection is a serious health problem worldwide. As the hepatitis B virus infects only humans and chimpanzees, establishment of reliable culture system is necessary to study this important pathogen. We cloned a fu11 length genotype C clone of the hepatitis B virus from a patient with a very mild hepatitis. About one and half genome was cloned into a plasmid vector. The 1.4 length clone was transfected to a HepG2 cell line. The resultant cell line produced HBs antigen, HBe antigen and HBV DNA to a supernatant. The titer of HBV DNA was 10^6-10^7 copy/ml. The production of the HBV DNA was reduced by incubation of lamivudine and interferons. The clones should be useful to further develop anti-hepatitis B virus agents and study biological properties of the hepatitis B virus.